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| Status |
Public on May 20, 2018 |
| Title |
Tissue-specific orchestration of nutrient dependent responses in D. melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Dietary restriction (DR) is a robust intervention to slow aging, but how individual tissues respond to DR is largely unknown. We developed a translating ribosome affinity purification method in Drosophila (Fly-TRAP) and investigated mRNA translational profiles of seven primary tissues upon DR.
Here we demonstrate that DR alters the mRNA translation expression of proteins genes involved in tissue structural integrity, metabolism, and signal communication in a tissue-specific and coordinated manner. DR enhances the expression of mitochondrial genes in some tissues but decreases in others. Genes involved in mRNA translation were increased specifically in the gut and fat body. A key finding from the study was that DR upregulates several neuroendocrine factors and their corresponding receptors in specific peripheral tissues. Additionally, several a list of secreted factors was in non-neuronal tissues were increased upon changed in response to DR, some of which affected longevity. These results suggest that DR enhances tissue-tissue communication to leading to a longer lifespan and improved tissue homeostasis.
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| Overall design |
82 experimental samples were analyzed using Nimblegen oligo microarrays. Cy3 single color array were used for both AL and DR samples.
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| Contributor(s) |
Du G, Li PW, Akagi K, Nelson C, Katewa SD, Tacutu R, Zykovich A, Melov S, Mooney SD, de Magalhães JP, Brem RB, Kapahi P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 13, 2016 |
| Last update date |
May 21, 2018 |
| Contact name |
Pankaj Kapahi |
| E-mail(s) |
pkapahi@buckinstitute.org
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| Organization name |
Buck Institute for Research on Aging
|
| Lab |
Pankaj Kapahi
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
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| Platforms (1) |
| GPL21873 |
NimbleGen Drosophila melanogaster 12X135K Gene Expression Array [100718_Dmel_exp; PROBE_ID version] |
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| Samples (82)
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| Relations |
| BioProject |
PRJNA321517 |
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