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Series GSE81317 Query DataSets for GSE81317
Status Public on Sep 26, 2017
Title Disruption of Trim9 function abrogates macrophage motility in vivo
Organism Danio rerio
Experiment type Expression profiling by array
Summary The vertebrate immune response is comprised of multiple molecular and cellular components that interface to provide an adequate defense against pathogens. Although much is known on how individual molecules or cells respond to infection, an understanding of the whole-organism response to pathogen exposure remains unresolved, due to the dynamic complexity of the immune system and its interdependent innate and adaptive functionality. Zebrafish larvae provide a unique model for overcoming this obstacle as larvae can successfully defends themselves from pathogens while lacking a functional adaptive immune system during the first few weeks of life, making it possible to examine exclusively the innate immune response in a whole-organism context. It was hypothesized that the transcriptional response of zebrafish larvae to immune agonists would identify known immune-response genes as well as reveal genes that mediate innate immunity in novel ways. In order to test this hypothesis, zebrafish larvae were exposure to the chemically diverse immune agonists, polyinosine-polycytidylic acid (PolyIC) and synthetic triacylated lipoprotein (Pam3CSK4) and transcriptome analyses completed using microarray analyses. This strategy successfully identified known immune response genes, as well as genes that had not been implicated in immune function, including the E3 ubiquitin ligase, tripartite motif 9 (trim9). Although Trim9 expression has been described as “brain specific”, here we demonstrate elevated levels of trim9 transcripts in macrophages after immune stimulation. As Trim9 has been implicated in axonal migration, we investigated and demonstrate that disruption of Trim9 function impairs macrophage chemotaxis and cellular architecture in vivo. These results demonstrate that Trim9 mediates cellular movement and migration in macrophages as well as neurons.
 
Overall design Zebrafish larvae were exposed to the chemically diverse immune agonists, polyinosine-polycytidylic acid (PolyIC) and synthetic triacylated lipoprotein (Pam3CSK4) and transcriptome analyses completed using microarray analyses. RNA were extracted at 4, 8, 12, 24 and 36 time points after exposure to PolyIC and Pam3CSK4. At each timepoint we have a untreated controls.
 
Contributor(s) Tokarz DA, Amy HK, Dereje JD, Jamie G, Radhika S, Ivan R, Ashley FA, Shila NK, J.Mac L, Steffen H, Jeffrey YA
Citation(s) 29021367
Submission date May 11, 2016
Last update date Jul 16, 2018
Contact name Dereje D. Jima
E-mail ddjima2014@gmail.com
Phone 9195155932
Organization name North Carolina State University
Department Center for Human Health and the Environment (CHHE) and Bioinformatics Research Center (BRC)
Street address 850 Campus Drive
City Raleigh
State/province NC
ZIP/Postal code 27708
Country USA
 
Platforms (1)
GPL21860 Agilent-015747 Zebrafish 44K Oligo Microarray (Probe name version)
Samples (52)
GSM2150227 Control_4hr_rep1
GSM2150228 Control_4hr_rep2
GSM2150229 Control_4hr_rep3
Relations
BioProject PRJNA321265

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Supplementary file Size Download File type/resource
GSE81317_RAW.tar 315.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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