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Series GSE81252 Query DataSets for GSE81252
Status Public on Feb 08, 2017
Title Multilineage communication regulates human liver bud self-organization from pluripotency I
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Conventional 2-D differentiation from pluripotency fails to recapitulate cell interactions occurring during organogenesis. 3-D organoids generate complex organ-like tissues, however it is unclear how heterotypic interactions impact lineage identity. Here we use single-cell RNA-seq to reconstruct hepatocyte-like lineage progression from pluripotency in 2-D culture. We then derive 3-D liver bud (LB) organoids by reconstituting hepatic, stromal, and endothelial interactions, and deconstruct heterogeneity during LB self-organization. We find that LB hepatoblasts differentiate towards hepatocyte fate, and in addition express epithelial migration signatures characteristic of organ budding. We identify hypoxia and inflammation signatures in endothelial and mesenchymal cells, which we suggest induce LB vasculogenesis. We use network analysis to predict autocrine and paracrine signaling in LBs, and show that VEGF crosstalk potentiates endothelial network formation and hepatoblast differentiation. Our molecular dissection reveals inter-lineage communication that is required for self-organization, and illuminates previously inaccessible aspects of human organ development and regeneration.
 
Overall design Single-cell transcriptomes from multiple time points during hepatocyte-like lineage progression from pluripotency in 2-D culture, and from 3-D liver bud (LB) organoids that self-organized after reconstituting hepatic, stromal, and endothelial interactions.
 
Contributor(s) Camp JG, Treutlein B
Citation(s) 27669147, 28614297
Submission date May 09, 2016
Last update date May 15, 2019
Contact name Gray Camp
E-mail(s) graycamp@gmail.com
Organization name Max Planck Institute for Evolutionary Anthropology
Department Evolutionary Genetics
Street address Deutscher Pl. 6
City Leipzig
ZIP/Postal code 04103
Country Germany
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (770)
GSM2170442 de_A10
GSM2170443 de_A11
GSM2170444 de_A2
Relations
BioProject PRJNA322308
SRA SRP075475

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE81252_data.cast.log2.lineage.csv.gz 23.0 Mb (ftp)(http) CSV
GSE81252_data.cast.log2.liverbud.csv.gz 23.5 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data is available on Series record

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