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Series GSE81193 Query DataSets for GSE81193
Status Public on May 31, 2016
Title Characterising the transcriptional profile of murine 3T3-L1 adipocytes with altered expression of IRF3.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The chronic inflammatory state that accompanies obesity is a major contributor to insulin resistance and other metabolic dysfunction features. Despite recent advances in our understanding of the cellular and secreted factors that promote the inflammatory milieu of obesity, we have much less insight into the transcriptional pathways that drive these processes. While most attention has focused on the canonical inflammatory transcription factor NF-KB, other potentially important factors exist, including members of the interferon regultory factor (IRF) family. Here we show that IRF3 expression is upregulated in the adipocytes of obese mice and humans. TLR3/TLR4 activation induces insulin resistance in adipocytes, which can be prevented by IRF3 knockdown. Furthermore, Irf3KO mice display improved insulin sensitivity, associated with reduced intra-adipose and systemic inflammation in the high-fat fed state, enhanced browning of subcutaneous fat, and increased adipose expression of Glut4. Taken together, our data indicates that IRF3 is a major transcriptional regulator of adipose inflammation to maintain systemic glucose and energy homeostasis.
Overall design Transcriptional profiling of murine 3T3-L1 adipocytes with altered expression of IRF3. Overexpression or knockdown of IRF3 was achieved by lentivirus transduction for 6 days. 3T3-L1 adipocytes with IRF3 knockdown were further treated in the absence or presence of LPS for 6 days. Samples consist of triplicate replica with appropriate control.
Contributor(s) Kumari M, Lyubetskaya A, Tenen D, Rosen ED
Citation(s) 27400129
Submission date May 06, 2016
Last update date May 15, 2019
Contact name Evan Rosen
Organization name Beth Israel Deconess Medical Center
Department Endocrinology
Lab Rosen Lab
Street address 3 Blackfan Cir
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (18)
GSM2144394 2D-rep1
GSM2144395 2D-rep2
GSM2144396 2D-rep3
BioProject PRJNA320871
SRA SRP074478

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Supplementary file Size Download File type/resource
GSE81193_raw_counts.txt.gz 2.6 Mb (ftp)(http) TXT
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