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Series GSE81112 Query DataSets for GSE81112
Status Public on May 04, 2017
Title Genome-wide location analysis of Abf1 and Reb1 in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Abf1 and Reb1, two general regulatory factors playing roles at promoters and other genome functional sites in budding yeast, were mapped genome-wide by ChIP-sequencing using strains expressing TAP-tagged versions of the proteins. As expected on the basis of previous in silico analysis of promoter regions, we found that these factors are enriched at the promoters of ribosome biogenesis (Ribi) genes, a large regulon of more than 200 genes required for ribosome biosynthesis and assembly, and known to be coordinately regulated in response to nutrient availability and cellular growth rate.
Overall design The reported analysis was designed as a confirmative experiment. Chromatin immunoprecipitation of TAP-tagged Abf1 and Reb1 was carried out using logarithmically growing yeast cells, as described in Fermi et al (2016) Nucleic Acids Res, March 25, doi:10.1093/nar/gkw194
Contributor(s) Bosio MC, Fermi B, Spagnoli G, Levati E, Rubbi L, Ferrari R, Pellegrini M, Dieci G
Citation(s) 28158860
Submission date May 04, 2016
Last update date May 15, 2019
Contact name Giorgio Dieci
Phone +390521905649
Organization name University of Parma
Department Deparment of Life Sciences
Street address Parco Area delle Scienze 23/A
City Parma
ZIP/Postal code 43124
Country Italy
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (4)
GSM2143113 ChIP for tagged-Abf1
GSM2143114 input for tagged-Abf1 strain
GSM2143115 ChIP for tagged-Reb1
BioProject PRJNA320553
SRA SRP074386

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Supplementary file Size Download File type/resource
GSE81112_RAW.tar 715.7 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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