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Series GSE80727 Query DataSets for GSE80727
Status Public on Mar 27, 2017
Title Utilizing single-cell variations to reveal environment-dependent tuning of network connectivity in human macrophages (RNA-Seq, CAGE and ChIP-Seq)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Through analysis of gene expression and chromatin state, this study examines human monocyte-derived macrophages activated in vitro by various stimuli such as cytokines or microbial products
 
Overall design Macrophages, differentiated for 6 days from human blood monocytes using M-CSF (100ng/mL) cultured in X-vivo 15, were treated with stimuli for 18 or 24 hours and RNA and Chromatin collected for RNAseq (Illumina Truseq Ribo Zero stranded and mRNA unstranded protocols), ChIPseq (H3K27ac), and ATACseq.
 
Contributor(s) Martins AJ, Narayanan M, Tsang JS
Citation(s) 28365150
BioProject PRJNA315538
Submission date Apr 27, 2016
Last update date May 15, 2019
Contact name Andrew James Martins
E-mail(s) andrew.martins@nih.gov
Phone 3017615289
Organization name National Institute of Allergy and Infectious Diseases
Department Laboratory of Immune System Biology
Lab Systems Genomics and Bioinformatics
Street address 4 Memorial Drive
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (29)
GSM2135456 140421_S2_CNT_A__
GSM2135457 140421_S2_CNT_B__
GSM2135458 140421_S2_IL10_A__
This SubSeries is part of SuperSeries:
GSE81444 Utilizing single-cell variations to reveal environment-dependent tuning of network connectivity in human macrophages
Relations
SRA SRP072029

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80727_IL10_RNAseq_countsForGEO.txt.gz 697.4 Kb (ftp)(http) TXT
GSE80727_cage_counts_forGEO.txt.gz 39.5 Kb (ftp)(http) TXT
GSE80727_h3k27ac_counts_forGEO.txt.gz 575.7 Kb (ftp)(http) TXT
GSE80727_mac_enhancers.bed.gz 76.9 Kb (ftp)(http) BED
GSE80727_union_H3K27ac_peakslax.bed.gz 749.9 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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