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| Status |
Public on Mar 27, 2017 |
| Title |
Utilizing single-cell variations to reveal environment-dependent tuning of network connectivity in human macrophages (RNA-Seq, CAGE and ChIP-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Through analysis of gene expression and chromatin state, this study examines human monocyte-derived macrophages activated in vitro by various stimuli such as cytokines or microbial products
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| |
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| Overall design |
Macrophages, differentiated for 6 days from human blood monocytes using M-CSF (100ng/mL) cultured in X-vivo 15, were treated with stimuli for 18 or 24 hours and RNA and Chromatin collected for RNAseq (Illumina Truseq Ribo Zero stranded and mRNA unstranded protocols), ChIPseq (H3K27ac), and ATACseq.
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| |
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| Contributor(s) |
Martins AJ, Narayanan M, Tsang JS |
| Citation(s) |
28365150 |
| BioProject |
PRJNA315538 |
| Submission date |
Apr 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew James Martins |
| E-mail(s) |
andrew.martins@nih.gov
|
| Phone |
3017615289
|
| Organization name |
National Institute of Allergy and Infectious Diseases
|
| Department |
Laboratory of Immune System Biology
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| Lab |
Systems Genomics and Bioinformatics
|
| Street address |
4 Memorial Drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (29)
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| This SubSeries is part of SuperSeries: |
| GSE81444 |
Utilizing single-cell variations to reveal environment-dependent tuning of network connectivity in human macrophages |
|
| Relations |
| SRA |
SRP072029 |
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