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Series GSE80698 Query DataSets for GSE80698
Status Public on May 07, 2016
Title Human Calu-3 cell transcriptome response to wild-type pandemic H1N1 (A/California/04/2009), natural isolate
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type pandemic H1N1 (A/California/04/2009), natural isolate.
 
Overall design Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Cells were infected with an MOI of 3. After infection, wash and replace with 2 ml of DMEM-F12 + .3% BSA and 1 ug/ml TPCK-trypsin. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 12, 24, and 36 hr post-infection.
 
Contributor(s) Walters K, Stratton K, Thompson A, Waters KM, Eisfeld AJ, Kawaoka Y
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Submission date Apr 26, 2016
Last update date Aug 31, 2016
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (29)
GSM2133721 ICL104_Mock_12hr_microRNA_1
GSM2133722 ICL104_Mock_12hr_microRNA_2
GSM2133723 ICL104_Mock_12hr_microRNA_3
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA319727

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80698_RAW.tar 102.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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