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Series GSE80697 Query DataSets for GSE80697
Status Public on May 26, 2016
Title Human Calu-3 cell transcriptome response to wild-type pandemic H1N1 (A/California/04/2009), natural isolate [mRNA]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type pandemic H1N1 (A/California/04/2009), natural isolate.
 
Overall design Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Cells were infected with an MOI of 3. After infection, wash and replace with 2 ml of DMEM-F12 + .3% BSA and 1 ug/ml TPCK-trypsin. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12, 24, 36, and 48 hr post-infection.
 
Contributor(s) Walters K, Stratton K, Thompson A, Waters KM, Eisfeld AJ, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 26, 2016
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (57)
GSM2133664 ICL104_Mock_0hr_RNA_1
GSM2133665 ICL104_Mock_0hr_RNA_2
GSM2133666 ICL104_Mock_0hr_RNA_3
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA319728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80697_RAW.tar 123.1 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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