NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE8059 Query DataSets for GSE8059
Status Public on Aug 07, 2007
Title resting and IL2 activated human natural killer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity .
Keywords: resting and IL2 activated NK cells(time series)
 
Overall design RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).
 
Contributor(s) Dybkaer K, Iqbal J, Zhou G, Geng H, Xiao L, Schmitz A, d’Amore F, Chan WC
Citation(s) 17623099
Submission date Jun 07, 2007
Last update date Jan 08, 2019
Contact name Javeed Iqbal
E-mail jiqbal@unmc.edu
Phone 402-559-7753
Organization name University of Nebraska Medical Center
Department Pathology and Microbiology
Lab Center for Research in Leukemia and Lymphoma
Street address 40th street
City omaha
State/province NE
ZIP/Postal code 68195
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM198942 Resting_NK
GSM198943 NK_time2hours
GSM198944 NK_timepoint8hours
Relations
BioProject PRJNA100891

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8059_RAW.tar 63.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap