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Series GSE80419 Query DataSets for GSE80419
Status Public on Mar 08, 2017
Title Il-22-Fc in cutaneous wound healing response
Organism Mus musculus
Experiment type Expression profiling by array
Summary Diabetic foot ulcers (DFU) are one of the major complications in type II diabetes patients and can result in amputation and morbidity. Although multiple approaches are used clinically to help wound closure, many patients still lack adequate treatment. Here we show that IL-20 subfamily cytokines are upregulated during normal wound healing. While there is a redundant role for each individual cytokine in this subfamily in wound healing, mice deficient in IL-22R, the common receptor chain for IL-20, IL-22, and IL-24, display a significant delay in wound healing. Furthermore, IL-20, IL-22 and IL-24 are all able to promote wound healing in type II diabetic db/db mice. When compared to other growth factors such as VEGF and PDGF that accelerate wound healing in this model, IL-22 uniquely induced genes involved in reepithelialization, tissue remodeling and innate host defense mechanisms from wounded skin. Interestingly, IL-22 treatment showed superior efficacy compared to PDGF or VEGF in an infectious diabetic wound model. Taken together, our data suggest that IL-20 subfamily cytokines, particularly IL-20, IL-22, and IL-24, might provide therapeutic benefit for patients with DFU.
Overall design There are 45 samples in total (nine groups n=5/group). We have prepared the RNA from Diabetic Foot Ulcer Skin wounds. The following is the experimental design: Groups 1-4: 24 hrs time point; Groups 5-9: day7 post wound; Group 1: db/db females, n=5, anti Ragweed; Group 2: db/db females, n=5 IL-22 Fc; Group 3: db/db females, n=5 VEGF; Group 4: db/db females, n=5 PDGF; Group 5: dbm females n=5, PBS (lean control mice); Group 6: db/db females, n=5, anti-Ragweed; Group 7: db/db females, n=5 IL-22 Fc; Group 8: db/db females, n=5 VEGF; Group 9: db/db females, n=5 PDGF anti-Ragweed (50 ug) and Il-22 Fc (20 ug). For groups 1-4, wound (6mm) will be created on Day0 and receive one dose of topical treatment. Mice will be euthanized 24 hrs post topical treatment and wound tissue will be harvested. For groups 5 through 9 wound will be created on day 0 and dosed topically on Day0, Day2, day4, day 6. Mice will be euthanized on Day 7 and wound tissue harvested and RNA prepared.
Contributor(s) Kolumam G, Wu X, Lee WP, Hackney JA, Zavala-Solorio J, Gandham V, Danilenko D, Arora P, Ouyang W, Wang X
Citation(s) 28125663
Submission date Apr 19, 2016
Last update date Feb 11, 2019
Contact name Mark McCreary
Phone 6502254332
Organization name Genentech
Department Bioinformatics
Street address 1 DNA Way, MS 93
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (42)
GSM2126937 SAM19826823
GSM2126938 SAM19826860
GSM2126939 SAM19826835
BioProject PRJNA318860

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Supplementary file Size Download File type/resource
GSE80419_RAW.tar 144.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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