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Series GSE80238 Query DataSets for GSE80238
Status Public on Sep 01, 2017
Title Gemone-wide changes of epigenetic mark H3K27 trimethylation (H3K27me3) and AR in prostate cancer cells upon the treatment of EZH2 inhibitors [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Our initial data showed different responses of abl and DU145 cells to EZH2 inhibitors, therefore we detected the chromatin binding signals of H3K27me3 in both cell lines in the presence of the compounds. We found that genome-wide changes in H3K27me3 levels were not associated with the inhibitor-mediated transcriptional programs or biological effects. Since the gene expression profiles upon the compound treatment indicated an important role of AR signaling in mediating the inhibitory action of the drugs in abl cells, we them mapped AR chromatin binding patterns in the presence or absence of EZH2 inhibitors. It was interesting to detect a dramatic decline in AR binding intesities at regulatory regions of the regulated target genes.
Overall design Detection of the changes in chromatin binding intesities/signals of H3K27me3 and AR upon EZH2 inhibitors in prostate cancer cells
Contributor(s) Xu K, Chen C
Citation(s) 35031563
Submission date Apr 13, 2016
Last update date Feb 08, 2022
Contact name Kexin Xu
Organization name UT Health Science Center at San Antonio
Department Molecular Medicine
Street address 7703 Floyd Curl, MC 8257
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM2122573 H3K27me3 ChIPSeq_abl_DMSO treated
GSM2122574 H3K27me3 ChIPSeq_abl_EPZ treated
GSM2122575 H3K27me3 ChIPSeq_abl_GSK treated
This SubSeries is part of SuperSeries:
GSE80240 Distinct changes in transcriptional profiles and epigenetic patterns mediated by EZH2 inhibitors in sensitive and insensitive prostate cancer cells
BioProject PRJNA318381
SRA SRP073246

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Supplementary file Size Download File type/resource
GSE80238_RAW.tar 2.3 Gb (http)(custom) TAR (of BED, BROADPEAK, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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