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| Status |
Public on Apr 13, 2016 |
| Title |
Transcriptome analysis in a radiosensitive and a radioresistant cell line after ionizing radiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Differential gene expression profiling was performed in two lymphoblastoid cell lines with different radiosentivitity, one radiosensitive (RS) and another radioresistant (RR), after different post-irradiation times. A greater and a prolonged transcriptional response after irradiation was induced in the RS cell line. Functional analysis showed that 24 h after irradiation genes involved in DNA damage response, negative regulation of the cell cycle and apoptosis were still differentially up-regulated in the RS cell line but not in the RR cell line.
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| Overall design |
Sham-irradiated and irradiated (2 Gy) cell cultures of the RS and the RR cell line were incubated at 37ºC for 4 and 24 h and 14 days. After that, RNA was extracted and sequenced with QuantSeq technology
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| Contributor(s) |
Borràs-Fresneda M, Barquinero JF, Gomolka M, Hornhardt S, Rössler U, Armengol G, Barrios L |
| Citation(s) |
27245205 |
| |
| Submission date |
Apr 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Mireia Borràs-Fresneda |
| E-mail(s) |
mireiaborras2@gmail.com
|
| Organization name |
Universitat Autònoma de Barcelona
|
| Street address |
s/n
|
| City |
Bellaterra |
| ZIP/Postal code |
08193 |
| Country |
Spain |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA318275 |
| SRA |
SRP073206 |
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