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Series GSE80059 Query DataSets for GSE80059
Status Public on May 07, 2016
Title Human liver Huh cell transcriptome (miRNA) response to a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The purpose is to obtain samples for microRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).
 
Overall design Human liver Huh cells were infected with a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants. The Huh7.0 VP30 cells were on split 5 for this experiment. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Two time courses were performed to generate samples for this experiment (the second set was started within 24 h of the first set). The time courses were 8 and 18 hours, and 24 and 48 hours.
 
Contributor(s) Halfmann P, Thompson A, Waters KM, Eisfeld AJ, Kawaoka Y
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Submission date Apr 08, 2016
Last update date Aug 31, 2016
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (78)
GSM2111915 EHUH001_mock_8h_1_microRNA
GSM2111916 EHUH001_mock_8h_3_microRNA
GSM2111917 EHUH001_mock_8h_4_microRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA317723

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80059_EHUH001_microRNA_GEO_matrix.xlsx 5.0 Mb (ftp)(http) XLSX
GSE80059_RAW.tar 268.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

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