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Series GSE80058 Query DataSets for GSE80058
Status Public on May 07, 2016
Title Human liver Huh cell transcriptome (mRNA) response to a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for mRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).
 
Overall design Human liver Huh cells were infected with a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants. The Huh7.0 VP30 cells were on split 5 for this experiment. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Two time courses were performed to generate samples for this experiment (the second set was started within 24 h of the first set). The time courses were 00, 8, and 18 hours, and 0, 24, and 48 hours.
 
Contributor(s) Halfmann P, Thompson A, Waters KM, Eisfeld AJ, Kawaoka Y
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Submission date Apr 08, 2016
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (119)
GSM2111796 EHUH001_mock_0h_1_RNA
GSM2111797 EHUH001_mock_0h_2_RNA
GSM2111798 EHUH001_mock_0h_3_RNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA317724

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE80058_EHUH001_mRNA_GEO_matrix.xlsx 56.3 Mb (ftp)(http) XLSX
GSE80058_RAW.tar 257.8 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

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