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Series GSE79887 Query DataSets for GSE79887
Status Public on Apr 05, 2016
Title Transcriptional profiling of IL-10 producing vs non producing human TFR2 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background information:
Antibody responses to most infectious and food protein antigens depend on help to B cells from specialised T follicular helper (Tfh) cells. A subset of Foxp3+ regulatory T cells (Tregs) has been described in mice, with a prominent role in repressing germinal center reactions that are critical for memory B cell formation and long-lived antibody responses. These specialised Tregs co-opt the Bcl-6-dependent Tfh differentiation pathway in order to access the B cell-rich follicles and have therefore been designated as T follicular regulatory (Tfr) cells.
Preliminary results:
We identified a unique Bcl-6-expressing follicular regulatory T cell in human secondary lymphoid tissue, designated hereafter as Tfr2 cells, that lacks Foxp3 expression and the thymic-imprinted Foxp3 methylation pattern, but shares expression of key Treg molecules. Interestingly, 25% of these cells are the predominant source of T cell-derived IL-10 in human tonsil, an important cytokine with immunomodulatory properties.
Aims of this study:
We performed transcriptional profiling using affymetrix microarrays to interrogate wether IL-10 producing vs non producing human Tfr2 cells were fundamentally different subsets.
 
Overall design 2 cell types (IL-10+ vs IL10- TFR2 cell subsets) obtained from 3 different tonsil donors (biological replicates)
 
Contributor(s) Sweet RA, Ohms S, Canete PF
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Submission date Apr 04, 2016
Last update date Mar 15, 2019
Contact name Pablo Fernandez De Canete Nieto
E-mail(s) pablo.fernandez@anu.edu.au
Organization name JCSMR,ANU
Street address building 131 garran road
City canberra
ZIP/Postal code 2601
Country Australia
 
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (6)
GSM2107853 IL-10 negative TFR2 cells 2
GSM2107854 IL-10 negative TFR2 cells 3
GSM2107855 IL-10 negative TFR2 cells 4
Relations
BioProject PRJNA317424

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79887_Differentially_expressed_genes.xlsx 29.8 Kb (ftp)(http) XLSX
GSE79887_RAW.tar 42.9 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

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