GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE79887 Query DataSets for GSE79887
Status Public on Apr 05, 2016
Title Transcriptional profiling of IL-10 producing vs non producing human TFR2 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background information:
Antibody responses to most infectious and food protein antigens depend on help to B cells from specialised T follicular helper (Tfh) cells. A subset of Foxp3+ regulatory T cells (Tregs) has been described in mice, with a prominent role in repressing germinal center reactions that are critical for memory B cell formation and long-lived antibody responses. These specialised Tregs co-opt the Bcl-6-dependent Tfh differentiation pathway in order to access the B cell-rich follicles and have therefore been designated as T follicular regulatory (Tfr) cells.
Preliminary results:
We identified a unique Bcl-6-expressing follicular regulatory T cell in human secondary lymphoid tissue, designated hereafter as Tfr2 cells, that lacks Foxp3 expression and the thymic-imprinted Foxp3 methylation pattern, but shares expression of key Treg molecules. Interestingly, 25% of these cells are the predominant source of T cell-derived IL-10 in human tonsil, an important cytokine with immunomodulatory properties.
Aims of this study:
We performed transcriptional profiling using affymetrix microarrays to interrogate wether IL-10 producing vs non producing human Tfr2 cells were fundamentally different subsets.
Overall design 2 cell types (IL-10+ vs IL10- TFR2 cell subsets) obtained from 3 different tonsil donors (biological replicates)
Contributor(s) Sweet RA, Ohms S, Canete PF
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 04, 2016
Last update date Mar 15, 2019
Contact name Pablo Fernandez De Canete Nieto
Organization name JCSMR,ANU
Street address building 131 garran road
City canberra
ZIP/Postal code 2601
Country Australia
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (6)
GSM2107853 IL-10 negative TFR2 cells 2
GSM2107854 IL-10 negative TFR2 cells 3
GSM2107855 IL-10 negative TFR2 cells 4
BioProject PRJNA317424

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79887_Differentially_expressed_genes.xlsx 29.8 Kb (ftp)(http) XLSX
GSE79887_RAW.tar 42.9 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap