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Series GSE79731 Query DataSets for GSE79731
Status Public on Sep 14, 2016
Title Gene expression data from Mycobacterium tuberculosis-infected WT and miR-155-/- bone-marrow derived macrophages [timecourse]
Organism Mus musculus
Experiment type Expression profiling by array
Summary How the complex interaction between Mycobacterium tuberculosis (Mtb) and the host is regulated during infection is still not well understood. Using a systems biology approach, we demonstrate here that miR-155 is one of several microRNAs that regulate host gene expression over the first 48 hours of Mtb infection in macrophages. miR-155 regulates the cell survival of Mtb-infected macrophages through SHIP1/AKT signaling.
Using timecourse gene expression data, we constructed a miRNA regulatory network for the innate immune response to Mtb infection by WT macrophages. The network suggested a role for seven miRNAs in regulating the host response to Mtb, with miR-155 being one of them. We then validated a role for miR-155 by comparing the response between WT and miR-155-/- macrophages.
 
Overall design Three replicates of WT C57BL/6 BMMs were collected at 0, 4, 8, 24, and 48 hours post-infection with Mycobacterium tuberculosis, H37Rv strain (MOI 5). The regulatory network was then validated with WT and miR-155-/- BMM samples collected at 0 and 8 hours post-infection (three replicates each).
This dataset represents three replicates of WT C57BL/6 BMMs were collected at 0, 4, 8, 24, and 48 hours post-infection with Mycobacterium tuberculosis, H37Rv strain (MOI 5).
 
Contributor(s) Sissons JR, Rothchild AC, Aderem A
Citation(s) 27681624
Submission date Mar 30, 2016
Last update date Dec 06, 2016
Contact name Aderem Lab
E-mail alan.diercks@seattlechildrens.org
Organization name Seattle Children's Research Institute
Lab Aderem Lab
Street address 307 Westlake Avenue North, Suite 500
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL13712 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [Alternative CDF: BrainArray version 14: MoEx10stv1_Mm_ENTREZG]
Samples (15)
GSM2101737 WT BMM, 0h, rep 1
GSM2101738 WT BMM, 0h, rep 2
GSM2101739 WT BMM, 0h, rep 3
This SubSeries is part of SuperSeries:
GSE79733 Gene expression data from Mycobacterium tuberculosis-infected WT and miR-155-/- bone-marrow derived macrophages
Relations
BioProject PRJNA316925

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79731_RAW.tar 392.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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