|
| Status |
Public on Sep 01, 2017 |
| Title |
Exploring the occupancy and interaction with FXR2, STAT1/3 and H3K4me3 at genomic level of FXR1 in H358 and AGS cell line by ChIP-seq |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) was conducted in the human cancer cell lines H358 and AGS using FXR1, FXR2, STAT1/3 and H3K4me3 specific antibodies on the platform Illumina HiSeq 2000. ChIP-seq data quality was analyzed using FastQC. Target protein binding genomic regions (called ChIP-seq peaks) were identified by Model-based Analysis of ChIP-Seq (MACS) algorithm using the default p-value cutoff of 1e-5.
|
| |
|
| Overall design |
ChIP-seq using antibodies against FXR1/FXR2/STAT1/STAT3/H3K4me3 in H358 and AGS cells
|
| |
|
| Contributor(s) |
Fan Y, Ma C, Yao Wang V, Li Y, Hua Y, Wen Z, Xiang B |
| Citation(s) |
28767039 |
| |
| Submission date |
Mar 30, 2016 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Bin Xiang |
| E-mail(s) |
bin.xiang@novartis.com
|
| Organization name |
novartis institutes for biomedical research
|
| Street address |
Building 3, 4218 Jinke Road, Pudong
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA316816 |
| SRA |
SRP072556 |
Less...
More...