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Series GSE79571 Query DataSets for GSE79571
Status Public on Mar 31, 2017
Title mRNA expression profiling of ebv-miR-BART7-expressing NPC cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Nasopharyngeal carcinoma (NPC) is endemic in Southeast Asia and southern China. The primary treatment for NPC is radiotherapy. Despite of the encouraging results of radiotherapy, local recurrence or distant metastases of NPC after the initial therapy is frequently found due to radioresistance. Therefore, there is an urgent need to identify genes that control radiosensitivty, aiming to reduce disease recurrence. Epstein-Barr virus (EBV) infection was closely associated with undifferentiated NPC. EBV-encoded microRNAs (miRNAs) played crucial roles in the pathogenesis of NPC. Ebv-miR-BART7 belongs to the 44 EBV BART miRNAs and was found to be up-regulated in NPC tissues and plasma. Forced expression of ebv-miR-BART7 enhanced the radiosensitivity of NPC cells. However, the mechanisms underlying the sensitizing effect of ebv-miR-BART7 on radiation remain largely unknown.
Given that miRNA exerts biological functions by regulating its targets, we used microarray to identify the targets of ebv-miR-BART7 that regulate radiosensitivity of NPC cells.
Overall design NPC HONE1 cells were transfected with ebv-miR-BART7 mimic or negative control siRNA. The experiment was done in duplicate. Then, HONE1 cells were irradiated at 4 gray. RNA were extracted and hybridized on Affymetrix microarrays.
Contributor(s) Wong T, Gao W
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Submission date Mar 24, 2016
Last update date Mar 25, 2019
Contact name Wei Gao
Organization name The University of Hong Kong
Street address 21 Sassoon Road
City Hong Kong
ZIP/Postal code Nill
Country Hong Kong
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (4)
GSM2098466 HONE1 Negative control, biological rep 1
GSM2098467 HONE1 Negative control, biological rep 2
GSM2098468 HONE1 ebv-miR-BART7, biological rep 1
BioProject PRJNA316199

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79571_RAW.tar 20.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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