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Series GSE79554 Query DataSets for GSE79554
Status Public on Nov 03, 2017
Title Targets of FRUITFULL in the pistil/silique [ChIP-Seq]
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To learn more about the role of FRUITFULL (FUL), in pistil/silique development, we performed a ChIP-seq experiment to identify direct targets of FUL in the pistil/silique.
Overall design We used a pFUL:FUL-GFP line to conduct chromatin immuno-precipitation experiments with GFP-specific antibodies followed by deep-sequencing (ChIP-seq) in order to determine FUL binding sites on a genome-wide scale. For the samples, pistils/siliques from flower stages 12-16 were harvested, fixed for 15-30’ and the immunoprecipitation was performed according to Kaufmann et al., 2010 (doi: 10.1038/ nprot.2009.244.). Experiments were done in two biological replicates with the according input samples as controls.
Contributor(s) Bemer M, van Mourik H, Angenent GC
Citation(s) 28586421
Submission date Mar 23, 2016
Last update date May 15, 2019
Contact name Marian Bemer
Organization name Wageningen University
Department Molecular Biology
Street address Droevendaalsesteeg 1
City Wageningen
ZIP/Postal code 6708 PB
Country Netherlands
Platforms (1)
GPL9302 Illumina Genome Analyzer II (Arabidopsis thaliana)
Samples (4)
GSM2098200 FUL-GFP in ful-1, replicate 1
GSM2098201 FUL-GFP in ful-1, replicate 2
GSM2098202 Input DNA of replicate 1 (GFF-1)
This SubSeries is part of SuperSeries:
GSE79555 Expression profiling and identification of targets of FRUITFULL in the Arabidopsis pistil/silique
BioProject PRJNA316152
SRA SRP072249

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79554_InputDNA_FULBindingSites_TAIR9.csv.gz 38.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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