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| Status |
Public on Nov 03, 2017 |
| Title |
Targets of FRUITFULL in the pistil/silique [ChIP-Seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To learn more about the role of FRUITFULL (FUL), in pistil/silique development, we performed a ChIP-seq experiment to identify direct targets of FUL in the pistil/silique.
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| Overall design |
We used a pFUL:FUL-GFP line to conduct chromatin immuno-precipitation experiments with GFP-specific antibodies followed by deep-sequencing (ChIP-seq) in order to determine FUL binding sites on a genome-wide scale. For the samples, pistils/siliques from flower stages 12-16 were harvested, fixed for 15-30’ and the immunoprecipitation was performed according to Kaufmann et al., 2010 (doi: 10.1038/ nprot.2009.244.). Experiments were done in two biological replicates with the according input samples as controls.
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| Contributor(s) |
Bemer M, van Mourik H, Angenent GC |
| Citation(s) |
28586421 |
| |
| Submission date |
Mar 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Marian Bemer |
| E-mail(s) |
marian.bemer@wur.nl
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| Organization name |
Wageningen University
|
| Department |
Molecular Biology
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| Street address |
Droevendaalsesteeg 1
|
| City |
Wageningen |
| ZIP/Postal code |
6708 PB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL9302 |
Illumina Genome Analyzer II (Arabidopsis thaliana) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE79555 |
Expression profiling and identification of targets of FRUITFULL in the Arabidopsis pistil/silique |
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| Relations |
| BioProject |
PRJNA316152 |
| SRA |
SRP072249 |
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