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Series GSE7951 Query DataSets for GSE7951
Status Public on Jun 30, 2007
Title Genome-wide gene expression profiling of rice stigma
Organism Oryza sativa
Experiment type Expression profiling by array
Summary In angiosperms, stigma provides initial nutrients and guidance cues for pollen grain germination and tube growth. However, little is known about genes that regulate these processes in rice. Here we generate rice stigma-specific gene expression profiles through comparing genome-wide expression patterns of hand dissected unpollinated stigma at anthesis with seven tissues including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds of five days after pollination, 10-day-old embryo, 10-day-old endosperm as well as suspension cultured cells by using 57K Affymetrix rice whole genome array. In total, we identified 665 probe sets (550 genes) to be expressed specifically or predominantly in the stigma papillar cells of rice. Real-Time quantitative RT-PCR analysis of 34 selected genes confirmed their stigma-specific expression. The expression of five selected genes was further validated by RNA in situ hybridization. Gene annotation shows that several auxin-signaling components, transporters and stress-related genes are significantly overrepresented in the rice stigma gene set. We also found that genes involved in cell wall metabolism and cellular communication appear to be conserved in the stigma between rice and Arabidopsis. Our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and Arabidopsis.
Keywords: rice (Oryza sativa L.), pollination and fertilization, stigma, molecular functions, signaling£¬microarray, stress response
 
Overall design We generate rice stigma-specific gene expression profiles through comparing genome-wide expression patterns of hand dissected unpollinated stigma at anthesis with seven tissues including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds of five days after pollination, 10-day-old embryo, 10-day-old endosperm as well as suspension cultured cells by using 57K Affymetrix rice whole genome array.
Web link http://plantbiol.genetics.ac.cn/rice-Affy/
 
Contributor(s) Li M, Xu W, Yang W, Kong Z, Xue Y
Citation(s) 17556504
Submission date May 29, 2007
Last update date Dec 27, 2017
Contact name Wenying Xu
E-mail(s) wyxu@genetics.ac.cn
Organization name Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
Street address Datun Road, Chaoyang District
City Beijing
ZIP/Postal code 100101
Country China
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (13)
GSM195218 stigma 1, biological rep1
GSM195219 stigma 2, biological rep 2
GSM195220 stigma 3, biological rep 2
Relations
BioProject PRJNA100133

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7951_RAW.tar 62.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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