The purpose is to obtain samples for microRNA analysis in primary human fibroblasts infected with wild type MERS-CoV (icMERS).
Human primary fibroblasts were infected with a multiplicity of infection of 5 PFU per cell. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 12, 24, and 36 h post-infection. MERSCOV003.0P references the write up of the experimental procedure.