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Series GSE79411 Query DataSets for GSE79411
Status Public on Aug 09, 2016
Title Analysis of melanosomal, exosomal and cellular miRNAs of melanoma cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary miRNA microarray profiling was performed for exosomes and secreted melanosomes of MNT-1 cells as well as WM3682 and WM3314 cells and melanosomes isolated from cell homogenates. The aim of this study is to compare the miRNA content of exosomes and secreted melanosomes and to assess differences in the miRNA profiles of different melanoma cell lines and their melanosomes.
 
Overall design Total RNA was extracted from MNT-1 exosomes and secreted melanosomes (2 replicates each) that were isolated from the conditioned medium of MNT-1 melanoma cells by differential ultracentrifugation. In addition, total RNA was isolated from WM3682 and WM3314 melanoma cells and melanosomes (1 replicate each). These latter melanosomes were isolated from cell homogenates by filtration through a 0.45 µm filter, followed by FACS sorting. Contributor: Microarray Unit of the Core Facility Genomics and Proteomics DKFZ
 
Contributor(s) Sander L, Dror S, Sébastien A, Levy C, Hoheisel JD
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Submission date Mar 20, 2016
Last update date Aug 09, 2016
Contact name Carmit Levy
E-mail(s) carmitlevy@post.tau.ac.il
Organization name Tel Aviv University
Department Human Genetics and Biochemistry
Lab Sackler School of Medicine
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL21576 Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (Probe Name version)
Samples (8)
GSM2094904 Exosomes MNT-1_rep1
GSM2094905 Exosomen_MNT-1_rep2
GSM2094906 Secreted melanosomes MNT-1_rep1
This SubSeries is part of SuperSeries:
GSE72620 Melanoma miRNA trafficking triggers the tumor primary niche formation
Relations
BioProject PRJNA315772

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79411_Normalized_data_with_all_controls.txt.gz 508.7 Kb (ftp)(http) TXT
GSE79411_RAW.tar 23.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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