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Series GSE79357 Query DataSets for GSE79357
Status Public on Feb 19, 2017
Title Polycomb-mediated deactivation of AR-feedback loop leads to castration-resistant prostate cancer [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Castration-resistant prostate cancer (CRPC) is a lethal disease, often characterized by aberrant androgen receptor (AR) activation independently of androgen. We have previously reported that AR can directly repress the expression of many target genes, one of which is NOV/CCN3. Here we show that NOV, primarily localized in the cytoplasm, physically interacts with AR at its Nterminus and sequesters AR and AR variants in the cytoplasm, thereby reducing AR chromatintargeting. This negative feedback loop, however, is disrupted in CRPC due to epigenetic silencing of NOV by the Polycomb group protein EZH2, rendering AR transcriptional activities and drug-resistant prostate cancer progression. Our findings thus suggest a working model wherein AR-repressed genes critically prevent CRPC through negative feedback loops inhibiting AR signaling.
 
Overall design ChIP_Seq examination of AR and NOV binding sites in LNCaP cells
 
Contributor(s) Yu J, Fong K, Zhao J
Citation(s) 27815387
Submission date Mar 17, 2016
Last update date May 15, 2019
Contact name Jindan Yu
E-mail(s) jindan-yu@northwestern.edu
Organization name Northwestern University
Department Division of Hematology/Oncology
Lab Jindan Yu's lab
Street address 303 E. Superior St., Lurie 5-117
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (3)
GSM2092844 LNCaP_Ctrl AR ChIP-seq
GSM2092845 LNCaP_NOV AR ChIP-seq
GSM2092846 LNCaP GIPZ Input
This SubSeries is part of SuperSeries:
GSE79358 Polycomb-mediated deactivation of AR-feedback loop leads to castration-resistant prostate cancer
Relations
BioProject PRJNA315572
SRA SRP071950

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79357_RAW.tar 102.5 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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