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Series GSE79227 Query DataSets for GSE79227
Status Public on Jan 17, 2017
Title Mammalian sperm exhibit epigenetic priming of embryonic and adult regulatory landscapes (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The mammalian sperm genome is generally thought to be condensed and transcriptionally inert. Here we show that most promoters in mouse sperm contain elongating RNA polymerase II and are flanked by several positioned nucleosomes marked by a variety of active histone modifications. The sperm genome is bound by several transcription factors, including Nanog, Oct4, Mediator, condensin, and the pioneer factor FoxA1. These proteins are found at promoters, enhancers, and super-enhancers, many of which are active in mESCs or adult tissues. CTCF and cohesin are also present in sperm DNA, where they mediate interactions that organize the sperm genome into domains and compartments that overlap extensively with those found in mESCs. These results indicate that the sperm genome is rich in epigenetic information and primed for its expression during embryonic and adult life, and suggest mechanisms by which environmental effects on the paternal germline can be transmitted trans-generationally.
Overall design Analysis of the distribution of histone modifications and chromatin proteins in mouse sperm
Contributor(s) Jung Y, Sauria M, Lyu X, Taylor J, Corces V
Citation(s) 28178516
Submission date Mar 15, 2016
Last update date May 15, 2019
Contact name Yoonhee Jung
Phone 4047274250
Organization name Emory university
Department Human Genetics
Lab Corces lab
Street address 615 Michael Street
City Atlanta
State/province Georgia
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (23)
GSM2088382 CTCF_ChIPSeq_replicate1
GSM2088383 CTCF_ChIPSeq_replicate2_technical
GSM2088384 CTCF_ChIPSeq_replicate3_biological
This SubSeries is part of SuperSeries:
GSE79230 Mammalian sperm exhibit epigenetic priming of embryonic and adult regulatory landscapes
BioProject PRJNA315210
SRA SRP071783

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Supplementary file Size Download File type/resource
GSE79227_RAW.tar 2.5 Gb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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