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Status |
Public on Jun 18, 2016 |
Title |
Demonstration of novel piRNA production in chromosome-fusion containing Oxytricha |
Platform organism |
Oxytricha trifallax |
Sample organisms |
Sterkiella histriomuscorum; Oxytricha trifallax |
Experiment type |
Non-coding RNA profiling by high throughput sequencing Third-party reanalysis
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Summary |
Oxytricha cells containing a chromosome fusion were backcrossed to wild-type, and the production of novel piRNAs spanning the fusion boundary was demonstrated by PIWI-protein (Otiwi-1) immunoprecipitation - seq
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Overall design |
RNA-IP of piRNAs from backcrossing Oxytricha cells, WT control was obtained from GSM860690.
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Contributor(s) |
Bracht JR, Wang X |
Citation(s) |
27267579 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
K22 CA184297 |
Model systems for the investigation of DNA methylation and drug repurposing |
AMERICAN UNIVERSITY |
Bracht |
F32 GM099462 |
Epigenetic regulation of programmed genome instability in O. trifallax |
PRINCETON UNIVERSITY |
Bracht |
R01 GM059708 |
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PRINCETON UNIVERSITY |
LANDWEBER |
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Submission date |
Mar 08, 2016 |
Last update date |
May 15, 2019 |
Contact name |
John Russell Bracht |
E-mail(s) |
jbracht@american.edu
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Organization name |
American University
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Department |
Biology
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Street address |
4400 Massachusetts Ave NW
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City |
Washington |
State/province |
DC |
ZIP/Postal code |
20016 |
Country |
USA |
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Platforms (1) |
GPL15927 |
Illumina Genome Analyzer IIx (Oxytricha trifallax) |
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Samples (1) |
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Relations |
Reanalysis of |
GSM860690 |
BioProject |
PRJNA314602 |
SRA |
SRP071298 |