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Series GSE78809 Query DataSets for GSE78809
Status Public on Sep 25, 2017
Title Novel neuroprotective and neurogenic phenotype of microglia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: Tissue macrophages contribute to development and protection, both requiring appropriately timed and located source(s) of factors controlling growth, cell differentiation and migration. Goal: To understand the role of microglia (tissue macrophages of the central nervous system), in promoting neurodevelopment and controlling neuroinflammation. Summary of findings: We show that microglia fulfill both these roles. In contrast to adult cells, neonatal microglia show a unique neurogenic phenotype with stem cell-like potential. Neonatal microglia are protective against neuroinflammation, and their transplantation ameliorates experimental autoimmune encephalomyelitis. A CD11c+ microglial subset predominates in primary myelinating areas of the developing brain and expresses genes for neuronal and glial survival, migration and differentiation. CD11c+ microglia are also found in clusters of repopulating microglia after experimental ablation and in neuroinflammation in adult mice, but despite some similarities, they do not recapitulate neurogenic neonatal microglia characteristics. Conclusions: We therefore identify a unique phenotype of neonatal microglia that deliver signals necessary for neurogenesis and myelination and suppress neuroinflammation.
Overall design The overall design was to compare transcriptomes of subsets of microglia isolated from neonatal mice, healthy adults, and adult mice with a neuroinflammatory disease (Experimental autoimmune encephalomyelitis, EAE), and to compare anti-inflammatory function of adult and neonatal microglia. Microglia were isolated by cell-sorting based on surface phenotype, and RNAseq data was analyzed using WGCNA, GO and DAVID approaches. Expression of selected genes and pathways was confirmed by histology and flow cytometry. Functional analysis involved transfer of isolated microglia to the central nervous system of animals with EAE and evaluation of outcome.
EAE = Experimental autoimmune encephalomyelitis
Contributor(s) Owens T, Holtman I
Citation(s) 28963396
Submission date Mar 01, 2016
Last update date May 15, 2019
Contact name Inge Holtman
Organization name UCSD
Department CMM
Lab Glass
Street address 9500 Gilman Drive
City La Jolla,
State/province California
ZIP/Postal code 92093
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (17)
GSM2078124 13_Neonates_CD11c_microglia
GSM2078127 2_EAE_CD11c_microglia
GSM2078128 1_EAE_CD11c_microglia
BioProject PRJNA313971
SRA SRP071039

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Supplementary file Size Download File type/resource
GSE78809_RAW.tar 2.4 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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