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Series GSE78284 Query DataSets for GSE78284
Status Public on Oct 25, 2016
Title Modeling the ESR1 tyrosine 537 mutation with CRISPR-Cas9 for mechanistic studies and evaluation of therapeutic approaches for metastatic breast cancer [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Estrogen receptor-α (ERα) is an important driver of breast cancer and is the target for hormonal therapies, anti-estrogens and drugs that limit estrogen biosynthesis (aromatase inhibitors). Mutations in the ESR1 gene identified in metastatic breast cancer provide a potential mechanism for acquired resistance to hormone therapies. We have used CRISPR-Cas9 mediated genome editing in the MCF-7 breast cancer cell line, generating MCF-7-Y537S. MCF-7-Y537S cells encode a wild-type (tyrosine 537) and a mutant (serine 537) allele. Growth of the line is estrogen-independent and expression of ERα target genes is elevated in the absence of estrogen. ER ChIP-seq was carried out to map global ERα binding sites in the presence and absence of estrogen. RNA-seq following estrogen treatment was used for gene expression analysis. We show that expression of ER target genes and ER recruitment to ER binding regions is similar in MCF-7 and MCF-7-Y537S cells, except that ER recruitment to DNA and expression of ER target genes is frequently elevated in the absence of estrogen
 
Overall design Hormone depleted MCF-7 LUC /Y537S mutant cells were treated with estrogen (10nM) or ETOH as vehicle control for 45 mins. Erα Chip-seq was performed using Illumnia methodology
 
Contributor(s) Ali S, Harrod A, Nguyen VT, Magnani L
Citation(s) 27748765
Submission date Feb 24, 2016
Last update date May 15, 2019
Contact name Van Thuy Mai Nguyen
E-mail(s) v.nguyen12@imperial.ac.uk
Organization name Imperial College London
Department Surgery and Cancer
Lab Simak Ali's lab
Street address Du Cane Road
City London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM2071220 Erα_Chipseq_MCF7LUC_INPUT
GSM2071221 Erα_Chipseq_MCF7LUC_ETOH_CHROMATIN
GSM2071222 Erα_Chipseq_MCF7LUC_E2_CHROMATIN
This SubSeries is part of SuperSeries:
GSE78286 Modeling the ESR1 tyrosine 537 mutation with CRISPR-Cas9 for mechanistic studies and evaluation of therapeutic approaches for metastatic breast cancer
Relations
BioProject PRJNA313092
SRA SRP070778

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78284_RAW.tar 1.5 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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