|
| Status |
Public on Sep 01, 2007 |
| Title |
The effect of erstressin and nostressin on cytokine-induced A172 human glioblastoma cells in culture. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The unfolded protein response (UPR), a signaling pathway triggered by endoplasmic reticulum (ER) stress, is induced by a range of environmental factors. Here we describe the identification and characterization of a synthetic small molecule, erstressin, which activates the UPR. Erstressin induced rapid phosphorylation of PERK and eIF2a and the alternative splicing of XBP-1, hallmark initiating events of the UPR. Further, erstressin activated the transcription of multiple genes involved in the UPR. Coincident with these effects, erstressin also downregulated the transcription of the inflammation-associated enzyme inducible nitric oxide synthase (iNOS) in cytokine-activated cells. A close analog of erstressin that failed to induce the UPR did not attenuate expression of iNOS, suggesting that both biological effects of erstressin were mediated by a common mechanism. Further, the structurally-distinct ER stressor thapsigargin also inhibited iNOS expression. Together these chemical genetic studies reveal an unanticipated anti-inflammatory role for the UPR.
Keywords: Time course
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| |
|
| Overall design |
We utilized microarrays to understand the global effect of a novel compund, erstressin, on cytokine stimulated cells over time.
|
| |
|
| Contributor(s) |
Dozier SJ, Hassig CA, Massari ME |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 15, 2007 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Sara Dozier |
| E-mail(s) |
saradozier@hotmail.com
|
| Organization name |
Kalypsys, Inc.
|
| Street address |
10420 Wateridge Circle
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (16)
|
| GSM189434 |
30min_nostressin_biological_rep_2 |
| GSM189435 |
6.5hr_erstressin_biological_rep_1 |
| GSM189436 |
6.5hr_erstressin_biological_rep_2 |
| GSM189437 |
6.5hr_nostressin_biological_rep_1 |
| GSM189438 |
6.5hr_nostressin_biological_rep_2 |
| GSM189439 |
18hr_erstressin_biological_rep_1 |
| GSM189440 |
18hr_erstressin_biological_rep_2 |
| GSM189441 |
18hr_nostressin_biological_rep_1 |
| GSM189442 |
18hr_nostressin_biological_rep_2 |
| GSM189443 |
18hr_erstressin_unstimulated_biological_rep_1 |
| GSM189444 |
18hr_erstressin_unstimulated_biological_rep_2 |
| GSM189445 |
6.5hr_vehicle_control_biological_rep_1 |
| GSM189446 |
6.5hr_vehicle_control_biological_rep_2 |
|
| Relations |
| BioProject |
PRJNA100023 |
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