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Series GSE77950 Query DataSets for GSE77950
Status Public on Aug 31, 2016
Title Long Non-coding RNA PICSAR Promotes Growth of Cutaneous Squamous Cell Carcinoma by Regulating ERK1/2 Activity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Keratinocyte-derived cutaneous squamous cell carcinoma (cSCC) is the most common metastatic skin cancer, and its incidence is increasing globally. Long non-coding RNAs (lncRNAs) are involved in various biological processes, and their role in cancer progression is emerging. Whole transcriptome analysis of cSCC cells (n=8) and normal human epidermal keratinocytes (NHEKs, n=4) revealed overexpression of long intergenic ncRNA (LINC00162) in cSCC cells (GSE66412). We wanted to futher study the RNA expression profile of LINC00162 knockdown cSCC cells. Based on our observations, LINC00162 was named PICSAR (P38 Inhibited Cutaneous Squamous cell carcinoma Associated lincRNA).
 
Overall design RNA-Seq analysis of cSCC cells followed by siRNA-induced gene knockdown of lincRNA PICSAR. Total RNAs from negative control and PICSAR siRNA-treated cSCC cell lines (n=3) were extracted. The samples were sequenced using Illumina sequencing.
 
Contributor(s) Piipponen M, Nissinen L, Kähäri V
Citation(s) 27049681, 30429154
Submission date Feb 16, 2016
Last update date May 15, 2019
Contact name Liisa Nissinen
E-mail(s) liisa.nissinen@utu.fi
Organization name University of Turku
Department Dermatology
Lab Cancer Research Laboratory
Street address Medisiina D, 5th floor; Kiinamyllynkatu 10
City Turku
ZIP/Postal code FI-20520
Country Finland
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM2062267 UT-SCC12A negative control siRNA
GSM2062268 UT-SCC12A PICSAR siRNA
GSM2062269 UT-SCC59A negative control siRNA
Relations
BioProject PRJNA312186
SRA SRP070156

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Supplementary file Size Download File type/resource
GSE77950_PICSAR_knockdown_in_cSCC_cells_Normalized_data_with_annotations.xlsx 2.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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