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Series GSE77944 Query DataSets for GSE77944
Status Public on Aug 17, 2016
Title Single-cell transcriptomes of each cell of the C. elegans embryo until the 16-cell stage
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary A prevalent hypothesis for the cell-to-cell coordination of the phenomena of early development is that a defined mixture of different mRNA species at specific abundances in each cell determines fate and behavior. With this dataset we explore this hypothesis by quantifying the abundance of every mRNA species in every individual cell of the early C. elegans embryo, for which the exact life history and fate is precisely documented.
 
Overall design Embryos of the 1-, 2-, 4-, 8- and 16-cell stage were dissected into complete sets of single cells, and each cell from each set was sequenced individually using SMARTer technology. 5-9 replicates were generated for each stage. Most cell identities were unknown upon sequencing, but were deduced from by their transcriptomes post hoc.
 
Contributor(s) Tintori SC, Osborne Nishimura E, Golden P, Lieb JD, Goldstein B
Citation(s) 27554860
Submission date Feb 16, 2016
Last update date May 15, 2019
Contact name Sophia C Tintori
E-mail(s) sophia.tintori@gmail.com
Organization name University of North Carolina - Chapel Hill
Street address CB #3280
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
Samples (219)
GSM2061813 st411_P0_1-cell_r1
GSM2061814 st413_P0_1-cell_r2
GSM2061815 st415_tossed_1-cell_r3
Relations
BioProject PRJNA312176
SRA SRP070155

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77944_RAW.tar 1.6 Gb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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