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Series GSE77924 Query DataSets for GSE77924
Status Public on Feb 16, 2016
Title Gene Expression Profiling of human monocytes: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transcriptome analysis of human peripheral blood monocytes
Combination therapy concurrently targeting PD-1 and CTLA-4 immune checkpoints leads to remarkable antitumor effects. Although both PD-1 and CTLA-4 dampen the T cell activation, the in vivo effects of these drugs in humans remain to be clearly defined. To better understand biologic effects of therapy, we analyzed blood/tumor tissue from patients undergoing single or combination immune checkpoint blockade. We show that blockade of CTLA-4, PD-1, or combination of the two leads to distinct genomic (changes in gene-expression profile) and functional signatures in vivo in purified human T cells and monocytes.
 
Overall design RNA extracted from freshly isolated monocytes from peripheral blood of patients treated with either anti–PD-1 (n = 6), anti–CTLA-4 (n = 5), Combo therapy with anti–PD-1 and anti–CTLA-4 concurrently (Combo, n = 6), and Seq anti–PD-1 in patients with prior anti–CTLA-4 (Seq, n = 3) was analyzed using the Affymetrix GeneChip Human Transcriptome 2.0 exon array. No techinical replicates were performed.
 
Contributor(s) Verma R
Citation(s) 25539810
Submission date Feb 15, 2016
Last update date Feb 17, 2016
Contact name Rakesh Verma
E-mail(s) rakesh.verma@yale.edu
Organization name Yale University
Department Yale Cancer Center
Lab Hematology
Street address 333 Cedar St.
City New Haven
State/province CT
ZIP/Postal code 06510
Country USA
 
Platforms (1)
GPL17585 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version]
Samples (39)
GSM2061400 Human Peripheral Blood monocytes [Sample_41]
GSM2061401 Human Peripheral Blood monocytes [Sample_42]
GSM2061402 Human Peripheral Blood monocytes [Sample_43]
Relations
BioProject PRJNA312113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77924_RAW.tar 968.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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