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Series GSE77714

Query DataSets for GSE77714

Status

Public on Feb 10, 2016

Title

Gene Expression Profiling of human T cells: Combination Therapy with Anti–CTLA-4 and Anti–PD-1 Leads to Distinct Immunologic Changes In Vivo

Organism

Experiment type

Expression profiling by array

Summary

Transcriptome analysis of human peripheral blood T cells
Combination therapy concurrently targeting PD-1 and CTLA-4 immune checkpoints leads to remarkable antitumor effects. Although both PD-1 and CTLA-4 dampen the T cell activation, the in vivo effects of these drugs in humans remain to be clearly defined. To better understand biologic effects of therapy, we analyzed blood/tumor tissue from patients undergoing single or combination immune checkpoint blockade. We show that blockade of CTLA-4, PD-1, or combination of the two leads to distinct genomic (changes in gene-expression profile) andfunctional signatures in vivo in purified human T cells.

Overall design

RNA extracted from freshly isolated T cells from peripheral blood of patients treated with either anti–PD-1 (n = 6), anti–CTLA-4 (n = 5), Combo therapy with anti–PD-1 and anti–CTLA-4 concurrently (Combo, n = 6), and Seq anti–PD-1 in patients with prior anti–CTLA-4 (Seq, n = 3) was analyzed using the Affymetrix GeneChip Human Transcriptome 2.0 exon array. No techinical replicates were performed.

Contributor(s)

Citation(s)

Submission date

Feb 09, 2016

Last update date

Feb 12, 2016

Contact name

Rakesh Verma

E-mail(s)

rakesh.verma@yale.edu

Organization name

Yale University

Department

Yale Cancer Center

Lab

Hematology

Street address

333 Cedar St.

City

New Haven

State/province

CT

ZIP/Postal code

06510

Country

USA

Platforms (1)

[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version]

Samples (40)

More...

GSM2056952	Human Peripheral Blood T cells [Sample_1]
GSM2056953	Human Peripheral Blood T cells [Sample_2]
GSM2056954	Human Peripheral Blood T cells [Sample_3]

GSM2056955	Human Peripheral Blood T cells [Sample_4]
GSM2056956	Human Peripheral Blood T cells [Sample_5]
GSM2056957	Human Peripheral Blood T cells [Sample_6]
GSM2056958	Human Peripheral Blood T cells [Sample_7]
GSM2056959	Human Peripheral Blood T cells [Sample_8]
GSM2056960	Human Peripheral Blood T cells [Sample_9]
GSM2056961	Human Peripheral Blood T cells [Sample_10]
GSM2056962	Human Peripheral Blood T cells [Sample_11]
GSM2056963	Human Peripheral Blood T cells [Sample_12]
GSM2056964	Human Peripheral Blood T cells [Sample_13]
GSM2056965	Human Peripheral Blood T cells [Sample_14]
GSM2056966	Human Peripheral Blood T cells [Sample_15]
GSM2056967	Human Peripheral Blood T cells [Sample_16]
GSM2056968	Human Peripheral Blood T cells [Sample_17]
GSM2056969	Human Peripheral Blood T cells [Sample_18]
GSM2056970	Human Peripheral Blood T cells [Sample_19]
GSM2056971	Human Peripheral Blood T cells [Sample_20]
GSM2056972	Human Peripheral Blood T cells [Sample_21]
GSM2056973	Human Peripheral Blood T cells [Sample_22]
GSM2056974	Human Peripheral Blood T cells [Sample_23]
GSM2056975	Human Peripheral Blood T cells [Sample_24]
GSM2056976	Human Peripheral Blood T cells [Sample_25]
GSM2056977	Human Peripheral Blood T cells [Sample_26]
GSM2056978	Human Peripheral Blood T cells [Sample_27]
GSM2056979	Human Peripheral Blood T cells [Sample_28]
GSM2056980	Human Peripheral Blood T cells [Sample_29]
GSM2056981	Human Peripheral Blood T cells [Sample_30]
GSM2056982	Human Peripheral Blood T cells [Sample_31]
GSM2056983	Human Peripheral Blood T cells [Sample_32]
GSM2056984	Human Peripheral Blood T cells [Sample_33]
GSM2056985	Human Peripheral Blood T cells [Sample_34]
GSM2056986	Human Peripheral Blood T cells [Sample_35]
GSM2056987	Human Peripheral Blood T cells [Sample_36]
GSM2056988	Human Peripheral Blood T cells [Sample_37]
GSM2056989	Human Peripheral Blood T cells [Sample_38]
GSM2056990	Human Peripheral Blood T cells [Sample_39]
GSM2056991	Human Peripheral Blood T cells [Sample_40]

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE77714_RAW.tar	1007.3 Mb	(http)(custom)	TAR (of CEL, CHP)
Processed data included within Sample table

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