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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 11, 2016 |
| Title |
Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis [microarray] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In the developing heart, heterotypic transcription factors (TFs) interactions, such as between the T-box TF TBX5 and the homeodomain TF NKX2-5 have been proposed as a mechanism for human congenital heart disease. In order to study the role of each TF during heart formation, embryonic stem (ES) cell-derived embryos were generated from KO ES cells for Tbx5, Nkx2-5 or both TFs.
We used microarrays to identify changes in the gene expression due to the lack of Tbx5, Nkx2-5 or both TFs during early heart formation.
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| Overall design |
WT, Nkx2-5KO (NKO), Tbx5KO (TKO) and Tbx5KO;Nkx2-5KO (DKO) E8.75 mouse hearts were microdissected for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Kaynak BL, Bruneau BG |
| Citation(s) |
26875865 |
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| Submission date |
Feb 04, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Benoit G. Bruneau |
| E-mail(s) |
bbruneau@gladstone.ucsf.edu
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| Organization name |
GLADSTONE INSTITUTES
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| Street address |
1650 OWENS STREET
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| City |
SAN FRANCISCO |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM1858054 |
E8.75 Nkx2-5 KO heart, biological rep1 |
| GSM1858055 |
E8.75 Nkx2-5 KO heart, biological rep2 |
| GSM1858056 |
E8.75 Nkx2-5 KO heart, biological rep3 |
| GSM1858057 |
E8.75 Tbx5 KO heart, biological rep1 |
| GSM1858058 |
E8.75 Tbx5 KO heart, biological rep2 |
| GSM1858059 |
E8.75 Tbx5 KO heart, biological rep3 |
| GSM1858060 |
E8.75 Tbx5 KO;Nkx2-5 KO heart, biological rep1 |
| GSM1858061 |
E8.75 Tbx5 KO;Nkx2-5 KO heart, biological rep2 |
| GSM1858062 |
E8.75 Tbx5 KO;Nkx2-5 KO heart, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE72223 |
Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis |
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| Relations |
| BioProject |
PRJNA310968 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE77576_RAW.tar |
61.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
| Processed data included within Sample table |
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