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Series GSE77576 Query DataSets for GSE77576
Status Public on Feb 11, 2016
Title Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis [microarray]
Organism Mus musculus
Experiment type Expression profiling by array
Summary In the developing heart, heterotypic transcription factors (TFs) interactions, such as between the T-box TF TBX5 and the homeodomain TF NKX2-5 have been proposed as a mechanism for human congenital heart disease. In order to study the role of each TF during heart formation, embryonic stem (ES) cell-derived embryos were generated from KO ES cells for Tbx5, Nkx2-5 or both TFs.
We used microarrays to identify changes in the gene expression due to the lack of Tbx5, Nkx2-5 or both TFs during early heart formation.
 
Overall design WT, Nkx2-5KO (NKO), Tbx5KO (TKO) and Tbx5KO;Nkx2-5KO (DKO) E8.75 mouse hearts were microdissected for RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Kaynak BL, Bruneau BG
Citation(s) 26875865
Submission date Feb 04, 2016
Last update date Mar 04, 2019
Contact name Benoit G. Bruneau
E-mail(s) bbruneau@gladstone.ucsf.edu
Organization name GLADSTONE INSTITUTES
Street address 1650 OWENS STREET
City SAN FRANCISCO
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM1858051 E8.75 WT heart, biological rep1
GSM1858052 E8.75 WT heart, biological rep2
GSM1858053 E8.75 WT heart, biological rep3
This SubSeries is part of SuperSeries:
GSE72223 Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis
Relations
BioProject PRJNA310968

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77576_RAW.tar 61.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data provided as supplementary file
Processed data included within Sample table

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