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Series GSE77564 Query DataSets for GSE77564
Status Public on May 26, 2016
Title Coupled electrophysiological recording and single-cell transcriptome analyses revealed molecular mechanisms underlying neuronal maturation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The mammalian brain is heterogeneous, containing billions of neurons and trillions of synapses forming various neural circuitries, through which sense, movement, thought, and emotion are generated. The cellular heterogeneity of the brain has made it difficult to study the molecular logic of neural circuitry wiring, pruning, activation, and plasticity, until recently, transcriptome analyses with single-cell resolution makes decoding of gene regulatory networks underlying aforementioned circuitry properties possible. Here, we report success in performing both electrophysiological and whole-genome transcriptome analyses on single human neurons in culture. Using Weighted Gene Coexpression Network Analyses (WGCNA), we identified gene clusters highly correlated with neuronal maturation judged by electrophysiological characteristics. A tight link between neuronal maturation and genes involved in ubiquitination and mitochondrial function was revealed. Moreover, we identified a list of candidate genes, which could potentially serve as biomarkers for neuronal maturation. Coupled electrophysiological recording and single-cell transcriptome analysis will serve as powerful tools in the future to unveil molecular logics for neural circuitry functions.
Overall design Patch-Seq of 20 hESC/hiPSC-derived neurons.
Contributor(s) Chen X, Zhang K, Zhou L, Gao X, Wang J, Yao Y, He F, Luo Y, Yu Y, Li S, Cheng L, Sun YE
Citation(s) 26883038
Submission date Feb 03, 2016
Last update date Mar 23, 2020
Contact name Kunshan Zhang
Organization name Tongji Hospital, Tongji University
Department Stem cell Translational Research center
Street address 389 Xincun Road
City Shanghai
ZIP/Postal code 200092
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (20)
GSM2054544 Single neuron 1
GSM2054545 Single neuron 2
GSM2054546 Single neuron 3
BioProject PRJNA310792
SRA SRP069264

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Supplementary file Size Download File type/resource
GSE77564_single_neuron_exp.txt.gz 865.9 Kb (ftp)(http) TXT
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