GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE77390 Query DataSets for GSE77390
Status Public on Dec 06, 2016
Title Probing Hemogenic Precursors with Endoglin Regulatory Elements uncovers LRP2 as a Regulator of Hematopoiesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Different combinations of Endoglin tissue specific enhancers define hemangioblast and hemogenic endothelium cell fractions
Overall design We generated a series of embryonic stem cell lines, each targeted with reporter constructs driven by tissue specific promoter/enhancer combinations of Endoglin (ENG). The Eng promoter (P) when combined with the -8/+7/+9kb enhancers targeted cells in FLK1 mesoderm that were enriched for blast colony forming potential, whereas the P/-8kb enhancer targeted TIE2+/c-KIT+/CD41- HE cells that were enriched for hematopoietic potential. These cell fractions were isolated and their transcriptomes profiled by RNA-seq.
Contributor(s) Nasrallah R, Beck D, Wong JW, Pimanda JE
Citation(s) 27554085
Submission date Jan 29, 2016
Last update date May 15, 2019
Contact name Dominik Beck
Organization name University of Technology Sydney
Department School of Biomedical Engineering
Street address Level 10, UTS Building 11 (CB11 81-113, Broadway, Ultimo)
City Sydney
State/province NSW
ZIP/Postal code 2007
Country Australia
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM2051232 23
GSM2051233 24
GSM2051234 110
BioProject PRJNA310206
SRA SRP069067

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77390_processed_data.txt.gz 459.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap