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| Status |
Public on Dec 06, 2016 |
| Title |
Probing Hemogenic Precursors with Endoglin Regulatory Elements uncovers LRP2 as a Regulator of Hematopoiesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Different combinations of Endoglin tissue specific enhancers define hemangioblast and hemogenic endothelium cell fractions
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| Overall design |
We generated a series of embryonic stem cell lines, each targeted with reporter constructs driven by tissue specific promoter/enhancer combinations of Endoglin (ENG). The Eng promoter (P) when combined with the -8/+7/+9kb enhancers targeted cells in FLK1 mesoderm that were enriched for blast colony forming potential, whereas the P/-8kb enhancer targeted TIE2+/c-KIT+/CD41- HE cells that were enriched for hematopoietic potential. These cell fractions were isolated and their transcriptomes profiled by RNA-seq.
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| |
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| Contributor(s) |
Nasrallah R, Beck D, Wong JW, Pimanda JE |
| Citation(s) |
27554085 |
| Submission date |
Jan 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Dominik Beck |
| E-mail(s) |
dominik.beck@uts.edu.au
|
| Organization name |
University of Technology Sydney
|
| Department |
School of Biomedical Engineering
|
| Street address |
Level 10, UTS Building 11 (CB11 81-113, Broadway, Ultimo)
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2007 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA310206 |
| SRA |
SRP069067 |
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