GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE77225 Query DataSets for GSE77225
Status Public on May 11, 2016
Title Negative control of CSL gene transcription by stress/DNA damage response and p53 [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary CSL is a key transcription factor, mostly acting as a repressor. While known as main effector of Notch signaling, it can also play Notch-independent functions. Despite the wide interest in CSL, the mechanisms responsible for its own regulation have been little studied. We recently showed that CSL down-modulation in human dermal fibroblasts (HDFs) leads to conversion into cancer associated fibroblasts, which promote keratinocyte tumor development. We show here that levels of CSL gene transcription differ among HDF strains derived from many different individuals, with negative correlation with genes involved in DNA damage/repair. CSL expression in all tested strains is negatively regulated by stress / DNA damaging insults caused by UVA, Reactive Oxygen Species (ROS), smoke extract and doxorubicin treatment. p53, a key effector of the DNA damage response, functions as common negative regulator of CSL gene transcription, through both suppression of CSL promoter activity and, indirectly, through increased p21 expression. CSL was previously shown to bind p53 suppressing its activity. The present findings indicate that p53, in turn, decreases CSL expression, which can serve to enhance p53 activity in the acute response of cells to DNA damaging cancer-threatening conditions.
Overall design ChIP sequencing of DMSO vs Nutlin-3a treated foreskin HDF
Contributor(s) Menietti E, Xu X, Ostano P, Joseph J, Lefort K, Dotto GP
Citation(s) 27163456
Submission date Jan 26, 2016
Last update date May 15, 2019
Contact name Gian-Paolo Dotto
Organization name UNIL
Department Biochemistry
Lab Dotto
Street address Chemin de Boveresses, 155
City Epalinges
ZIP/Postal code 1066
Country Switzerland
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM2046275 EM1
GSM2046276 EM2
GSM2046277 EM3
This SubSeries is part of SuperSeries:
GSE77371 Negative control of CSL gene transcription by stress/DNA damage response and p53
BioProject PRJNA309779
SRA SRP068925

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77225_DMSO_macs14_peaks.txt.gz 303.6 Kb (ftp)(http) TXT
GSE77225_EM2_vs_EM1_annotated_macs14_peaks-2015-05-29-160935.csv.gz 1.3 Mb (ftp)(http) CSV
GSE77225_EM4_vs_EM3_annotated_macs14_peaks-2015-05-29-161244.csv.gz 2.3 Mb (ftp)(http) CSV
GSE77225_Nutlin_macs14_peaks.txt.gz 548.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap