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Series GSE77197 Query DataSets for GSE77197
Status Public on Mar 17, 2016
Title Signaling networks of developing hair follicles
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The hair follicle (HF) is a complex miniorgan that serves as an ideal model system to study stem cell (SC) interactions with the niche during growth and regeneration. Dermal papilla (DP) cells are required for activating SCs during the adult hair cycle, but the signal exchange between niche and SC precursors/transit amplifying progenitor cells (TACs) that regulates HF morphogenetic growth is largely unknown. Here we use six transgenic reporters to isolate 14 major skin and HF cell populations. With next-generation RNA sequencing we characterize their transcriptomes and define unique molecular signatures. SC precursors, TACs and the DP niche express a plethora of known and novel ligands and receptors. Signaling interaction network analysis reveals a birds-eye view of pathways implicated in epithelial-mesenchymal interactions. Using a systematic tissue-wide approach this work provides a comprehensive platform, linked to an interactive online database, to identify and further explore the SC/TAC/niche crosstalk regulating HF growth.
 
Overall design FACS was used to isolate specific cell types from P5 mouse back skin
 
Contributor(s) Rezza A, Wang Z, Rendl M
Citation(s) 27009580
Submission date Jan 25, 2016
Last update date Mar 19, 2019
Contact name Michael Rendl
E-mail michaelrendl@gmail.com
Organization name Mount Sinai School of Medicine
Street address 1428 Madison Avenue
City New York
State/province NY
ZIP/Postal code 10029
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (28)
GSM2045898 P5 back skin_Epi_1
GSM2045899 P5 back skin_ORS_1
GSM2045900 P5 back skin_Mx_1
Relations
BioProject PRJNA309686
SRA SRP068850

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77197_repFpkmMatrix_P5_back_skin.txt.gz 1.7 Mb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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