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Series GSE77089 Query DataSets for GSE77089
Status Public on Mar 31, 2018
Title Transcriptomic responses of Clostridium (Ruminiclostridium) thermocellum ATCC27405 and ethanol tolerant strains to ethanol stress
Organism Acetivibrio thermocellus ATCC 27405
Experiment type Expression profiling by array
Summary Transcriptome profiles for Clostridium thermocellum ATCC 27405 wild type strain and two ethanol-adapted strains, E50A and E50C were generated to gain insights into ethanol tolerance. Details of the strains have been described, Shao X., et al. Appl Microbiol Biotechnol (2011) 92:641–652.
 
Overall design Duplicate fermentations using MTC medium were conducted for Clostridium thermocellum strains, ATCC 27405 wild-type; ethanol adapted strain E50A (adapted to grow in presence of up to 50 g/L ethanol on Avicel); and ethanol adapted strain E50C (adapted to grow in presence of up to 50 g/L ethanol on cellobiose). These strains have been described previously by Shao X., et al Appl Microbiol Biotechnol (2011) 92:641–652. A fifty two array study using total RNA processed from fermenation cultures of Clostridium thermocellum ATCC 27405 and ethanol adapted strains E50C and E50A, which were shocked with either 10 or 40 g/L ethanol. Cells were harvested immediately after, 15 minutes, 1 hour, 2 hours, and 4 hours after ethanol shock.
 
Contributor(s) Shao X, Raman B, Johnson CM, Rodriguez M, Mielenz JR, Lynd LR, Brown SD
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Submission date Jan 21, 2016
Last update date Apr 01, 2018
Contact name Jason Whitham
E-mail(s) whithamjm@ornl.gov
Organization name Oak Ridge National Labs
Street address 1 Bethel Valley Rd Bld 1505 Rm 366 MS 6038
City Oak Ridge
ZIP/Postal code 37831
Country USA
 
Platforms (1)
GPL18170 NimbleGen Clostridium thermocellum ATCC 27405 HX12 array (12 x 135k)(non-condensed version) [080724_C_thermo_27405_expr]
Samples (52)
GSM2043770 ATCC 27405_Avicel_Ethanol 10g/L_0.25PT [390218_A10_RL_FH_grid]
GSM2043771 ATCC 27405_Avicel_Ethanol 10g/L_0.25PT [389237_A3_RL_FH_grid]
GSM2043772 ATCC 27405_Avicel_Ethanol 10g/L_0.25PT [390155_1_Dartmouth#2_grid]
Relations
BioProject PRJNA309774

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77089_RAW.tar 110.4 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
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