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Series GSE76973 Query DataSets for GSE76973
Status Public on Mar 07, 2018
Title Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Gametes are highly specialised cells that can give rise to the next generation through their ability to generate a totipotent zygote. In mouse, germ cells are first specified in the developing embryo as primordial germ cells (PGCs) starting around embryonic day (E) 6.25. Following subsequent migration into the developing gonad, PGCs undergo a wave of extensive epigenetic reprogramming at E10.5/E11.5, including genome-wide loss of 5-methylcytosine (5mC). The underlying molecular mechanisms of this process have remained enigmatic leading to our inability to recapitulate this step of germline development in vitro. Using an integrative approach, we show that this complex reprogramming process involves the coordinated interplay between promoter sequence characteristics, DNA (de)methylation, Polycomb (PRC1) complex and both DNA demethylation-dependent and -independent functions of Tet1 to enable the activation of a critical set of germline reprogramming responsive (GRR) genes involved in gamete generation and meiosis. Our results also unexpectedly reveal a role for Tet1 in safeguarding but not driving DNA demethylation in gonadal PGCs. Collectively, our work uncovers a fundamental biological role for gonadal germline reprogramming and identifies the epigenetic principles of the PGC-to-gonocyte transition that will be instructive towards recapitulating complete gametogenesis in vitro.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Citation(s) 29513657
Submission date Jan 19, 2016
Last update date May 15, 2019
Contact name Peter Hill
E-mail peter.hill1@imperial.ac.uk
Organization name Imperial College London
Street address Kensington
City London
ZIP/Postal code SW7 2AZ
Country United Kingdom
 
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (69)
GSM2041475 E10.5 PGC AbaSeq Replicate 1
GSM2041476 E10.5 PGC AbaSeq Replicate 2
GSM2041477 E11.5 PGC AbaSeq Replicate 1
This SuperSeries is composed of the following SubSeries:
GSE76957 Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition (AbaSeq)
GSE76958 Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition (RNA-Seq)
GSE76962 Epigenetic reprogramming enables the primordial germ cell-to-gonocyte transition (RRBS)
Relations
BioProject PRJNA309236

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76973_RAW.tar 395.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp

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