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Series GSE76599 Query DataSets for GSE76599
Status Public on Jan 30, 2016
Title Human Calu-3 cell transcriptome response to a wild type infectious clone of H5N1 Influenza virus and mutant H5N1 viruses [mRNA]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with wild-type A/Vietnam/1203/2004 (H5N1; 'VN1203'; derived by reverse genetics), PB2-K627E (627K is a mammalian-adapting mutation that increases polymerase replicatve activity; '627E' is typically found in avian species), and NS1-trunc124 (this 'NS1' protein has a 90 amino acid truncation at the C-terminus).
 
Overall design Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12, and 24 hr post-infection.
 
Contributor(s) Walters K, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 06, 2016
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (80)
GSM2028768 ICL103_Mock_0hr_1
GSM2028769 ICL103_Mock_0hr_2
GSM2028770 ICL103_Mock_0hr_3
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA308121

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76599_ICL103_mRNA_GEO_matrix.xlsx 39.4 Mb (ftp)(http) XLSX
GSE76599_RAW.tar 172.4 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

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