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Series GSE76566 Query DataSets for GSE76566
Status Public on Dec 19, 2016
Title Chemical inhibitors of the Mycobacterium tuberculosis DosRST two-component regulatory system
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study was to discover and characterize small molecule inhibitors of the M. tuberculosis DosRST two-component regulatory pathway. The impact of three novel DosRST regulon inhibitors on gene expression was studied in wild type M. tuberculosis and a DosRS mutant strain.
Overall design Mtb strain CDC1551 or the CDC1551 (DdosRS mutant) was grown in standing T-25 flasks in 8 mL of medium seeded an initial OD of 0.1. Cultures were treated with 40 mM of HC101A, HC102A, HC103A or a DMSO control. Following 6 days of incubation at 37C, RNA was isolated from the bacterial cultures and used for RNA-seq.
Contributor(s) Zheng H, Johnson BK, Colvin CJ, Abramovitch RB
Citation(s) 27992879
Submission date Jan 05, 2016
Last update date Sep 06, 2018
Contact name Robert B. Abramovitch
Organization name Michigan State University
Department Microbiology and Molecular Genetics
Street address 567 Wilson Road
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
Platforms (1)
GPL17879 Illumina HiSeq 2500 (Mycobacterium tuberculosis)
Samples (16)
GSM2028027 WT treated with DMSO, rep 1
GSM2028028 WT treated with DMSO, rep 2
GSM2028029 WT treated with HC101A, rep 1
BioProject PRJNA307990
SRA SRP068117

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76566_TableS4A_WT_DMSO_VS_DOSR_DMSO.csv.gz 213.6 Kb (ftp)(http) CSV
GSE76566_TableS4B_WT_HC101A_VS_WT_DMSO.csv.gz 221.4 Kb (ftp)(http) CSV
GSE76566_TableS4C_WT_HC102A_VS_WT_DMSO.csv.gz 200.1 Kb (ftp)(http) CSV
GSE76566_TableS4D_WT_HC103A_VS_WT_DMSO.csv.gz 209.4 Kb (ftp)(http) CSV
GSE76566_TableS4E_DOSR_HC101A_VS_DOSR_DMSO.csv.gz 205.5 Kb (ftp)(http) CSV
GSE76566_TableS4F_DOSR_HC102A_VS_DOSR_DMSO.csv.gz 280.8 Kb (ftp)(http) CSV
GSE76566_TableS4G_DOSR_HC103A_VS_DOSR_DMSO.csv.gz 206.1 Kb (ftp)(http) CSV
Raw data are available in SRA
Processed data is available on Series record

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