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Series GSE76512 Query DataSets for GSE76512
Status Public on Jan 05, 2016
Title Type I Interferons Control Proliferation and Function of Intestinal Epithelium
Organism Mus musculus
Experiment type Expression profiling by array
Summary Wnt pathway-driven proliferation and renewal of the intestinal epithelium must be tightly controlled to prevent development of cancer and barrier dysfunction. Although type 1 interferons (IFN) produced in the gut under influence of microbiota are known for their anti-proliferative effects, the role of these cytokines in regulating intestinal epithelial renewal is largely unknown. Here we report a novel role for IFN in the context of intestinal knockout of casein kinase 1α (CK1α), which controls ubiquitination and degradation of both β-catenin and the IFNAR1 chain of the IFN receptor. Ablation of CK1α leads to activation of both β-catenin and IFN pathway and prevents unlimited proliferation of intestinal epithelial cells despite constitutive β-catenin activity. IFN signaling contributes to activation of the p53 pathway and appearance of apoptotic and senescence markers in the CK1α-deficient gut. Concurrent genetic ablation of CK1α and IFNAR1 leads to intestinal hyperplasia, robust attenuation of apoptosis, and rapid and lethal loss of the barrier function. These data indicate that IFN plays an important role in controlling proliferation and function of intestinal epithelium in the context of β-catenin activation.
 
Overall design Two conditions were examined, with 2 replicates for each condition, yielding 4 samples in total.
 
Contributor(s) Beiting D, Fuchs S, Katlinskaya Y
Citation(s) 26811327
Submission date Jan 04, 2016
Last update date Jun 14, 2018
Contact name daniel beiting
E-mail beiting@vet.upenn.edu
Organization name University of Pennsylvania
Department Pathobiology
Lab Beiting
Street address 380 S. University Ave
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM2027322 WT_colon_1
GSM2027323 WT_colon_2
GSM2027324 IFNAR1ko_colon_1
Relations
BioProject PRJNA307598

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76512_Non-normalized_data.txt.gz 497.0 Kb (ftp)(http) TXT
GSE76512_RAW.tar 3.1 Mb (http)(custom) TAR
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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