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Series GSE76447 Query DataSets for GSE76447
Status Public on Jan 01, 2016
Title Transcriptome profiling of post-mature green seeds from Arabidopsis ddcc mutant and wild-type
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The role of on-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the transcriptome of ddcc mutant in Arabidopsis post-mature green seeds using Illumina sequencing. Meanwhile, post-mature green seeds from wild type were used as control.
 
Overall design Illumina sequencing of transcripts from post-mature green seeds of ddcc mutant and wild type. Two biological replicates were collected.
 
Contributor(s) Goldberg RB, Lin J, Chen M
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Submission date Dec 31, 2015
Last update date Dec 23, 2018
Contact name Bob Goldberg
E-mail bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (4)
GSM2024779 ddcc mutant post-mature green seeds biological replicate 1
GSM2024780 ddcc mutant post-mature green seeds biological replicate 2
GSM2024781 wild type post-mature green seeds biological replicate 1
Relations
BioProject PRJNA307336
SRA SRP067963

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76447_RAW.tar 630.0 Kb (http)(custom) TAR (of TXT)
Raw data are available in SRA
Processed data provided as supplementary file

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