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Series GSE76428 Query DataSets for GSE76428
Status Public on Dec 31, 2015
Title Gene expression data from adipocytes of insulin resistant mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary We developed a novel network inference approach, Biologically Anchored Knowledge Expansion (BAKE), to analyze large volume gene expression data obtained from a mouse model of insulin resistance progression.
Both genetic aspects and dietary factors, specifically high caloric high-fat high-sugar diets, contribute to the progression of insulin resistance. To mimic genetic predisposition, we used a mouse model with double heterozygous deletion of early insulin signaling pathway intermediates, insulin receptor (IR) and insulin receptor substrate 1 (IRS1) genes. These mice were fed with high-fat (Western) or low-fat (Chow) diet for 8 and 16 weeks starting at 8 weeks of age. Gene expression data was collected from adipocytes isolated from these mice. Applying BAKE analysis to the adipocyte gene expression data, we demonstrate that we can accurately discover a novel regulatory gene in the insulin signaling pathway.
 
Overall design The mouse model of double heterozygous deletion of insulin receptor (IR) and insulin receptor substrate 1 (IRS1) was originally introduced as a polygenic model to study the development of type 2 diabetes. This mouse model, on an atherosclerosis-prone ApoE null background (IR+/- IRS1+/- ApoE-/-), also shows increased atherosclerotic lesions due to impaired insulin signaling. For our study we used female double heterozygous mice (IR+/- IRS1+/-, 'Dhet' mice or 'Dā€™ mice) on an ApoE null background (ApoE-/-, ā€˜Eā€™) fed with a Western (high-fat) diet for 8 (DW8, n=5) and 16 (DW16, n=9) weeks starting at 8 weeks of age or with a Chow (low-fat) diet (DC8, n=7; DC16, n=5). There were also ApoE null mice (ApoE-/-, 'Eā€™) fed either Western diet for 8 (EW8, n=6) and 16 (EW16, n=8) weeks or Chow diet for 16 weeks (EC16, n=5) starting at 8 weeks of age.
 
Contributor(s) Jae LK, Susanna KR
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Submission date Dec 30, 2015
Last update date Feb 11, 2019
Contact name Annamalai Muthiah
E-mail am2ta@virginia.edu
Phone 5404420248
Organization name University of Virginia
Street address 857, Swan Ridge Road
City Charlottesville
State/province VA
ZIP/Postal code 22903
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (45)
GSM2011457 Adipocytes_Dhet.mice_Western.diet_8weeks, rep1
GSM2011458 Adipocytes_Dhet.mice_Western.diet_8weeks, rep2
GSM2011459 Adipocytes_Dhet.mice_Western.diet_8weeks, rep3
Relations
BioProject PRJNA307265

Download family Format
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Supplementary file Size Download File type/resource
GSE76428_RAW.tar 169.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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