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| Status |
Public on Sep 26, 2016 |
| Title |
lncRNA DINO ChIRPseq in DNA damaged U2OS cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Examine the chormatin localization of DINO in DNA damaged human U2OS cells using DINO ChIRP-seq.
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| Overall design |
DINO is a DNA damage induced long noncoding RNA required for the transactivation of several p53-regulated genes. We examined the chormatin localization of DINO in DNA damaged human U2OS cells in order to determine whether DINO regulated gene expression by interacting with gene regulatory elements. We performed ChIRP with two independed probe sets (even and odd) according to previously published methods (Chu et al. 2011) . RNAse treated chromatin controls identify we utilized to confirm that DINO ChIRP recovery of DNA was indeed RNA dependent and not the result of direct DNA interaction by the probes.
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| Contributor(s) |
Schmitt A, Shen Y, Chang H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Adam Schmitt |
| E-mail(s) |
amschmit@stanford.edu
|
| Phone |
650-736-0305
|
| Fax |
650-723-8762
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| Organization name |
Stanford University
|
| Department |
Department of Dermatology
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| Lab |
Howard Y. Chang
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5168 |
| Country |
USA |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE76420 |
LncRNA DINO guides DNA damage signaling |
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| Relations |
| BioProject |
PRJNA307254 |
| SRA |
SRP067918 |
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