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| Status |
Public on Oct 06, 2016 |
| Title |
Single Cell RNA-seq Study of Midbrain and Dopaminergic Neuron Development in Mouse, Human, and Stem Cells |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to get a better molecular understanding of human midbrain development, this study defines cell types of the ventral midbrain in both human and mouse as well as stem cell-derived dopaminergic neuron preparations, and reveals the temporal dynamics of key lineages across development and the adult.
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| Overall design |
Single cells according to Samples list consisting of: Human embryo ventral midbrain cells between 6 and 11 weeks of gestation, mouse ventral midbrain cells at six developmental stages between E11.5 to E18.5, Th+ neurons at P19-P27, and putative dopaminergic neurons at P28-P56 FACS-sorted from Slc6a3-Cre/tdTomato mice. Stem cells (iPSCs and ESCs) preparation at different stages of differentiation towards Th+ neurons.
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| Contributor(s) |
LaManno G, Gyllborg D, Arenas E, Linnarsson S |
| Citation(s) |
27716510, 30655290 |
| |
| Submission date |
Dec 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sten Linnarsson |
| Organization name |
Karolinska Institutet
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| Department |
Medical Biochemistry and Biophysics
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| Lab |
Molecular Neurobiology
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| Street address |
Scheeles väg 1
|
| City |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6179)
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| Relations |
| BioProject |
PRJNA307121 |
| SRA |
SRP067844 |
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