|
| Status |
Public on Jan 01, 2016 |
| Title |
METTL3 promotes translation in human cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
we find METTL3 associates with polyribosomes and promotes translation. METTL3 depletion inhibits translation, and both wild-type and catalytically inactive METTL3 promote translation when tethered to the 3' untranslated region (UTR) of a reporter mRNA. Mechanistically, METTL3 enhances mRNA translation through an interaction with the translation initiation machinery.
|
| |
|
| Overall design |
m6A seq in A549 and H1299 cells, RNA seq in METTL3 knockdown cells
|
| |
|
| Contributor(s) |
Gregory R, Lin S |
| Citation(s) |
27117702 |
| Submission date |
Dec 28, 2015 |
| Last update date |
May 27, 2020 |
| Contact name |
Peng Du |
| E-mail(s) |
Peng.Du@childrens.harvard.edu
|
| Organization name |
Boston Children’s Hospital, Harvard Medical School
|
| Lab |
Richard Gregory
|
| Street address |
1 Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA307106 |
| SRA |
SRP067836 |
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