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Series GSE76267 Query DataSets for GSE76267
Status Public on Oct 12, 2016
Title The germ cell fate of cynomolgus monkeys is specified in the amnion
Organism Macaca fascicularis
Experiment type Expression profiling by high throughput sequencing
Summary The germ cell lineage ensures reproduction, heredity and evolution. However, the mechanism for germ cell specification in primates, including humans, has been unknown. In primates, the pluripotent epiblast segregates the amnion upon implantation and thereafter initiates gastrulation to generate three germ layers. Here, we show that in cynomolgus monkeys, the SOX17/BLIMP1/TFAP2C-positive primordial germ cells (cyPGCs) arise from the dorsal amnion at embryonic day (E) 11. cyPGCs then migrate down beneath the epiblast and expand their numbers, through proliferation and continuous recruitment from the posterior amnion, around posterior yolk sac endoderm by E17. Remarkably, the amnion itself expresses BMP4, a cytokine potentially critical for PGC specification, thereby inducing cyPGCs in an autocrine fashion. Consistently, the amnion expresses T, a key mesodermal factor, prior to the onset of gastrulation. Our study demonstrates the origin of cyPGCs in the amnion, which undergoes a unique morphogenetic event prior to and independent from the gastrulation.
Overall design Single cell transcriptome analysis of cynomolgus monkey embryo E13-20 using SC3-seq technology.
Contributor(s) Sasaki K, Nakamura T, Okamoto I, Yabuta Y, Iwatani C, Tsuchiya H, Shiraki N, Takakuwa T, Yamamoto T, Saitou M
Citation(s) 27720607, 28649393
Submission date Dec 22, 2015
Last update date May 15, 2019
Contact name Yukihiro Yabuta
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
Platforms (1)
GPL19944 AB 5500xl Genetic Analyzer (Macaca fascicularis)
Samples (34)
GSM1978209 E20_MS08T78
GSM1978210 E20_MS08T79
GSM1978211 E20_MS08T81
BioProject PRJNA306752
SRA SRP067695

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Supplementary file Size Download File type/resource
GSE76267_expression_RPM.txt.gz 2.9 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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