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Series GSE76130 Query DataSets for GSE76130
Status Public on Feb 18, 2016
Title Alternative routes to induced pluripotent stem cells revealed by reprogramming of neural lineage
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During the reprogramming of mouse embryonic fibroblasts (MEFs) to induced pluripotent stem cells, the activation of pluripotency genes such as Nanog occurs after the mesenchymal to epithelial transition (MET). Here we report that both adult stem cells (neural stem cells- NSCs) and differentiated cells (astrocytes) of the neural lineage can activate Nanog in the absence of cadherin expression during reprogramming. Gene expression analysis revealed that only the Nanog+Ecadherin+ populations expressed stabilization markers and had upregulated several cell cycle genes; and were transgene independent. Inhibition of Dot1L activity, which has previously been shown to increase MET, enhanced both the numbers of Nanog+ and Nanog+E-cadherin+ colonies, suggesting a MET independent pathway for activation of Nanog in NSCs. Expressing Sox2 in MEFs prior to reprogramming does not alter the ratio of Nanog colonies that express E-cadherin obtained. Taken together these results provide a novel pathway for reprogramming taken by cells of the neural lineage.
Overall design Neural Stem Cells (NSCs) were induced to reprogram by the induction of Oct4, Sox2, c-Myc and Klf4. Reprogramming colonies that expressed either Nanog alone (N+E-) or Nanog and E-cadherin (N+E+) were sorted by flow cytometry and used as input for RNA-sequencing. There are 3 replicates each for the N+E- and N+E+ samples.
Contributor(s) Sridharan R
Citation(s) 26905202
Submission date Dec 17, 2015
Last update date May 15, 2019
Contact name Rupa Sridharan
Organization name University of Wisconsin
Department Department of Regenerative Biology
Street address 330 N. Orchard Street
City Madison
State/province WI
ZIP/Postal code 53715
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM1974843 Sample1_Rep1_Nanog+E-cadherin+
GSM1974844 Sample2_Rep1_Nanog+E-cadherin-
GSM1974845 Sample3_Rep2_Nanog+E-cadherin+
BioProject PRJNA306377
SRA SRP067534

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Supplementary file Size Download File type/resource
GSE76130_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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