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Series GSE76074 Query DataSets for GSE76074
Status Public on May 05, 2017
Title Distinct Chromatin States Define a Program Of Mesenchymal Stem Cell Commitment To Osteogenesis
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Multipotent mesenchymal stromal cells (MSCs) from bone marrow are critical for regeneration and homeostasis of multiple tissues. As epigenetic mechanisms are a fundamental regulator of lineage specification and cell fate, we examined histone modification changes in MSCs at distinct stages osteogenic differentiation, including: proliferation, early commitment, matrix deposition, and mineralization. Temporal changes of multiple histone modifications along with several transcriptional regulators were assessed and correlated to gene expression, which revealed distinct epigenetic mechanisms that regulate transcriptional programs necessary for commitment and tissue-specific phenotype development. Patterns of stage-specific enrichment of histone modifications revealed distinct modes of repression and activation of gene expression that would not be detected using single endpoint analysis. In addition, we have defined dynamic enhancer regions that control tissue-specific genes. Our work provides a cornerstone to understand the epigenetic regulation of transcriptional programs that are important for MSC commitment, as well as insights to facilitate MSC-based therapeutic interventions.
 
Overall design ChIP-seq

Raw data are not available for sample GSM1973717.
 
Contributor(s) Wu H, Gordon J, Boyd J, Whitfield T, Lian J
Citation(s) 28077316
Submission date Dec 16, 2015
Last update date May 15, 2019
Contact name Jonathan AR Gordon
E-mail(s) Jonathan.A.Gordon@uvm.edu
Organization name University of Vermont
Department Biochemistry
Street address 89 Beaumont Ave Given E209
City Burlington
State/province VT
ZIP/Postal code 05405
Country USA
 
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (80)
GSM1973715 BMSC_d00_ctcf_R1
GSM1973716 BMSC_d00_ctcf_R2
GSM1973717 BMSC_d07_ctcf_R1
Relations
BioProject PRJNA306161
SRA SRP067461

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76074_ctcf_bmsc_combined_bigwigs.tar.gz 6.1 Gb (ftp)(http) TAR
GSE76074_h3k27ac_bmsc_combined_bigwigs.tar.gz 4.2 Gb (ftp)(http) TAR
GSE76074_h3k27me3_bmsc_combined_bigwigs.tar.gz 3.5 Gb (ftp)(http) TAR
GSE76074_h3k36me3_bmsc_combined_bigwigs.tar.gz 3.8 Gb (ftp)(http) TAR
GSE76074_h3k4me3_bmsc_combined_bigwigs.tar.gz 2.0 Gb (ftp)(http) TAR
GSE76074_h3k4me_bmsc_combined_bigwigs.tar.gz 5.0 Gb (ftp)(http) TAR
GSE76074_h3k9ac_bmsc_combined_bigwigs.tar.gz 3.0 Gb (ftp)(http) TAR
GSE76074_h3k9me3_bmsc_combined_bigwigs.tar.gz 3.4 Gb (ftp)(http) TAR
GSE76074_runx2_bmsc_combined_bigwigs.tar.gz 3.5 Gb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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